METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE

No significant change was found in the electrolytes and lipids of the brain analyzed after glutaraldehyde fixation by perfusion of laboratory animals; such fixation also satisfactorily preserves neural tissues for electron microscopy. The brains of normal and tumor-bearing C3H mice, Wistar rats, and...

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Detalles Bibliográficos
Autores principales: Levy, W. Arnoldo, Herzog, Ivan, Suzuki, Kunihiko, Katzman, Robert, Scheinberg, Labe
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1965
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106809/
https://www.ncbi.nlm.nih.gov/pubmed/19866694
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author Levy, W. Arnoldo
Herzog, Ivan
Suzuki, Kunihiko
Katzman, Robert
Scheinberg, Labe
author_facet Levy, W. Arnoldo
Herzog, Ivan
Suzuki, Kunihiko
Katzman, Robert
Scheinberg, Labe
author_sort Levy, W. Arnoldo
collection PubMed
description No significant change was found in the electrolytes and lipids of the brain analyzed after glutaraldehyde fixation by perfusion of laboratory animals; such fixation also satisfactorily preserves neural tissues for electron microscopy. The brains of normal and tumor-bearing C3H mice, Wistar rats, and New Zealand rabbits were studied. Little difference was found in the dry weight and the content of sodium, potassium, total lipid and lipid fractions, and in the sulfate space (S(35)O(4)) between specimens from unperfused and perfused animals, whether normal or tumor-bearing. The results suggest the possibility of using selected regions of the nervous system, dissected after fixation, for chemical study and at the same time characterizing similar regions morphologically with the electron microscope.
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spelling pubmed-21068092008-05-01 METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE Levy, W. Arnoldo Herzog, Ivan Suzuki, Kunihiko Katzman, Robert Scheinberg, Labe J Cell Biol Article No significant change was found in the electrolytes and lipids of the brain analyzed after glutaraldehyde fixation by perfusion of laboratory animals; such fixation also satisfactorily preserves neural tissues for electron microscopy. The brains of normal and tumor-bearing C3H mice, Wistar rats, and New Zealand rabbits were studied. Little difference was found in the dry weight and the content of sodium, potassium, total lipid and lipid fractions, and in the sulfate space (S(35)O(4)) between specimens from unperfused and perfused animals, whether normal or tumor-bearing. The results suggest the possibility of using selected regions of the nervous system, dissected after fixation, for chemical study and at the same time characterizing similar regions morphologically with the electron microscope. The Rockefeller University Press 1965-10-01 /pmc/articles/PMC2106809/ /pubmed/19866694 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Levy, W. Arnoldo
Herzog, Ivan
Suzuki, Kunihiko
Katzman, Robert
Scheinberg, Labe
METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title_full METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title_fullStr METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title_full_unstemmed METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title_short METHOD FOR COMBINED ULTRASTRUCTURAL AND BIOCHEMICAL ANALYSIS OF NEURAL TISSUE
title_sort method for combined ultrastructural and biochemical analysis of neural tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106809/
https://www.ncbi.nlm.nih.gov/pubmed/19866694
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