STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted...

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Detalles Bibliográficos
Autores principales: ChoraŻy, M., Bendich, A., Borenfreund, E., Hutchison, D. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106868/
https://www.ncbi.nlm.nih.gov/pubmed/14069802
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author ChoraŻy, M.
Bendich, A.
Borenfreund, E.
Hutchison, D. J.
author_facet ChoraŻy, M.
Bendich, A.
Borenfreund, E.
Hutchison, D. J.
author_sort ChoraŻy, M.
collection PubMed
description A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted and the chromosomes isolated by a series of differential centrifugations in sucrose. The isolated chromosomes were well preserved, as judged by morphological criteria. The effect of various enzymes and chemical agents on the isolated chromosomes was studied. Chymotrypsin, trypsin, and deoxyribonuclease caused a marked disintegration of the chromosomes, whereas treatment with pepsin and ribonuclease induced no significant morphological alterations.
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spelling pubmed-21068682008-05-01 STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES ChoraŻy, M. Bendich, A. Borenfreund, E. Hutchison, D. J. J Cell Biol Article A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted and the chromosomes isolated by a series of differential centrifugations in sucrose. The isolated chromosomes were well preserved, as judged by morphological criteria. The effect of various enzymes and chemical agents on the isolated chromosomes was studied. Chymotrypsin, trypsin, and deoxyribonuclease caused a marked disintegration of the chromosomes, whereas treatment with pepsin and ribonuclease induced no significant morphological alterations. The Rockefeller University Press 1963-10-01 /pmc/articles/PMC2106868/ /pubmed/14069802 Text en Copyright © 1963 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
ChoraŻy, M.
Bendich, A.
Borenfreund, E.
Hutchison, D. J.
STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title_full STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title_fullStr STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title_full_unstemmed STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title_short STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES
title_sort studies on the isolation of metaphase chromosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106868/
https://www.ncbi.nlm.nih.gov/pubmed/14069802
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AT borenfreunde studiesontheisolationofmetaphasechromosomes
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