BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria

Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are...

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Detalles Bibliográficos
Autores principales: Stahl, W. L., Smith, J. C., Napolitano, L. M., Basford, R. E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1963
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106877/
https://www.ncbi.nlm.nih.gov/pubmed/14090748
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author Stahl, W. L.
Smith, J. C.
Napolitano, L. M.
Basford, R. E.
author_facet Stahl, W. L.
Smith, J. C.
Napolitano, L. M.
Basford, R. E.
author_sort Stahl, W. L.
collection PubMed
description Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucrose. The data on enzymic properties and contamination by non-mitochondrial material indicate that mitochondrial fractions which compare favorably with those from other tissues can be prepared from brain tissue.
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spelling pubmed-21068772008-05-01 BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria Stahl, W. L. Smith, J. C. Napolitano, L. M. Basford, R. E. J Cell Biol Article Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucrose. The data on enzymic properties and contamination by non-mitochondrial material indicate that mitochondrial fractions which compare favorably with those from other tissues can be prepared from brain tissue. The Rockefeller University Press 1963-11-01 /pmc/articles/PMC2106877/ /pubmed/14090748 Text en Copyright © 1963 by The Rockefeller Institute Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stahl, W. L.
Smith, J. C.
Napolitano, L. M.
Basford, R. E.
BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title_full BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title_fullStr BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title_full_unstemmed BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title_short BRAIN MITOCHONDRIA : I. Isolation of Bovine Brain Mitochondria
title_sort brain mitochondria : i. isolation of bovine brain mitochondria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106877/
https://www.ncbi.nlm.nih.gov/pubmed/14090748
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AT smithjc brainmitochondriaiisolationofbovinebrainmitochondria
AT napolitanolm brainmitochondriaiisolationofbovinebrainmitochondria
AT basfordre brainmitochondriaiisolationofbovinebrainmitochondria

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