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INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES
The incorporation of thymidine-H(3) and lysine-H(3) into human leukocyte chromosomes was studied in order to determine the temporal relationships between the syntheses of chromosomal deoxyribonucleic acid and chromosomal protein. The labeled compounds were incorporated into nuclei of interphase cell...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1966
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106901/ https://www.ncbi.nlm.nih.gov/pubmed/5962297 |
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author | Cave, Mac Donald |
author_facet | Cave, Mac Donald |
author_sort | Cave, Mac Donald |
collection | PubMed |
description | The incorporation of thymidine-H(3) and lysine-H(3) into human leukocyte chromosomes was studied in order to determine the temporal relationships between the syntheses of chromosomal deoxyribonucleic acid and chromosomal protein. The labeled compounds were incorporated into nuclei of interphase cells. Label from both precursors became apparent over the chromosomes of dividing cells. Incorporation of thymidine-H(3) occurred during a restricted period of midinterphase (S) which was preceded by a nonsynthetic period (G(1)) and followed by a nonsynthetic period (G(2)). Incorporation of lysine-H(3) into chromosomal protein occurred throughout interphase. Grain counts made over chromosomes of dividing cells revealed that the rate of incorporation of lysine-H(3) into chromosomal protein differed during various periods of interphase. The rate of incorporation was diminished during G(1). During early S period the rate of incorporation increased, reaching a peak in late S. The high rate continued into G(2). Thymidine-H(3) incorporated into DNA was distributed to mitotic chromosomes of daughter cells in a manner which has been referred to as a "semi-conservative segregation." No such semi-conservative mechanism was found to affect the distribution of lysine-H(3) to the mitotic chromosomes of daughter cells. Therefore, it is concluded that synthesis of chromosomal protein and its distribution to chromosomes of daughter cells are not directly influenced by synthesis and distribution of the chromosomal DNA with which the protein is associated. |
format | Text |
id | pubmed-2106901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1966 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21069012008-05-01 INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES Cave, Mac Donald J Cell Biol Article The incorporation of thymidine-H(3) and lysine-H(3) into human leukocyte chromosomes was studied in order to determine the temporal relationships between the syntheses of chromosomal deoxyribonucleic acid and chromosomal protein. The labeled compounds were incorporated into nuclei of interphase cells. Label from both precursors became apparent over the chromosomes of dividing cells. Incorporation of thymidine-H(3) occurred during a restricted period of midinterphase (S) which was preceded by a nonsynthetic period (G(1)) and followed by a nonsynthetic period (G(2)). Incorporation of lysine-H(3) into chromosomal protein occurred throughout interphase. Grain counts made over chromosomes of dividing cells revealed that the rate of incorporation of lysine-H(3) into chromosomal protein differed during various periods of interphase. The rate of incorporation was diminished during G(1). During early S period the rate of incorporation increased, reaching a peak in late S. The high rate continued into G(2). Thymidine-H(3) incorporated into DNA was distributed to mitotic chromosomes of daughter cells in a manner which has been referred to as a "semi-conservative segregation." No such semi-conservative mechanism was found to affect the distribution of lysine-H(3) to the mitotic chromosomes of daughter cells. Therefore, it is concluded that synthesis of chromosomal protein and its distribution to chromosomes of daughter cells are not directly influenced by synthesis and distribution of the chromosomal DNA with which the protein is associated. The Rockefeller University Press 1966-05-01 /pmc/articles/PMC2106901/ /pubmed/5962297 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Cave, Mac Donald INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title | INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title_full | INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title_fullStr | INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title_full_unstemmed | INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title_short | INCORPORATION OF TRITIUM-LABELED THYMIDINE AND LYSINE INTO CHROMOSOMES OF CULTURED HUMAN LEUKOCYTES |
title_sort | incorporation of tritium-labeled thymidine and lysine into chromosomes of cultured human leukocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106901/ https://www.ncbi.nlm.nih.gov/pubmed/5962297 |
work_keys_str_mv | AT cavemacdonald incorporationoftritiumlabeledthymidineandlysineintochromosomesofculturedhumanleukocytes |