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RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE

Fixation by osmium tetroxide and glutaraldehyde of zymogen granules isolated from rat parotid and pancreas was investigated. Protein determinations showed that osmium tetroxide caused rapid release of most of the soluble protein of the granule during fixation in buffered isotonic sucrose. Such granu...

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Detalles Bibliográficos
Autores principales: Amsterdam, Abraham, Schramm, Michael
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106903/
https://www.ncbi.nlm.nih.gov/pubmed/5335826
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author Amsterdam, Abraham
Schramm, Michael
author_facet Amsterdam, Abraham
Schramm, Michael
author_sort Amsterdam, Abraham
collection PubMed
description Fixation by osmium tetroxide and glutaraldehyde of zymogen granules isolated from rat parotid and pancreas was investigated. Protein determinations showed that osmium tetroxide caused rapid release of most of the soluble protein of the granule during fixation in buffered isotonic sucrose. Such granules when examined in the electron microscope after shadow casting appeared quite flat, indicating that most of the contents had indeed been removed. Numerous damaged membranes of the granules were also observed. In contrast, zymogen granules fixed by glutaraldehyde and shadow cast essentially retained the spherical shape and the protein contents. The application of the shadow-casting technique in quantitative studies on the protein content of zymogen granules is discussed.
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spelling pubmed-21069032008-05-01 RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE Amsterdam, Abraham Schramm, Michael J Cell Biol Article Fixation by osmium tetroxide and glutaraldehyde of zymogen granules isolated from rat parotid and pancreas was investigated. Protein determinations showed that osmium tetroxide caused rapid release of most of the soluble protein of the granule during fixation in buffered isotonic sucrose. Such granules when examined in the electron microscope after shadow casting appeared quite flat, indicating that most of the contents had indeed been removed. Numerous damaged membranes of the granules were also observed. In contrast, zymogen granules fixed by glutaraldehyde and shadow cast essentially retained the spherical shape and the protein contents. The application of the shadow-casting technique in quantitative studies on the protein content of zymogen granules is discussed. The Rockefeller University Press 1966-05-01 /pmc/articles/PMC2106903/ /pubmed/5335826 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Amsterdam, Abraham
Schramm, Michael
RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title_full RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title_fullStr RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title_full_unstemmed RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title_short RAPID RELEASE OF THE ZYMOGEN GRANULE PROTEIN BY OSMIUM TETROXIDE AND ITS RETENTION DURING FIXATION BY GLUTARALDEHYDE
title_sort rapid release of the zymogen granule protein by osmium tetroxide and its retention during fixation by glutaraldehyde
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106903/
https://www.ncbi.nlm.nih.gov/pubmed/5335826
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