FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS

A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achi...

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Detalles Bibliográficos
Autores principales: Packer, Lester, Nobel, Park S., Gross, Elizabeth L., Mel, Howard C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106942/
https://www.ncbi.nlm.nih.gov/pubmed/5960807
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author Packer, Lester
Nobel, Park S.
Gross, Elizabeth L.
Mel, Howard C.
author_facet Packer, Lester
Nobel, Park S.
Gross, Elizabeth L.
Mel, Howard C.
author_sort Packer, Lester
collection PubMed
description A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effects; (c) the fractions are collected continuously, with size fractionation being evidenced; and (d) particles are separated into fractions of higher and lower activities as compared with the control population.
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spelling pubmed-21069422008-05-01 FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS Packer, Lester Nobel, Park S. Gross, Elizabeth L. Mel, Howard C. J Cell Biol Article A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effects; (c) the fractions are collected continuously, with size fractionation being evidenced; and (d) particles are separated into fractions of higher and lower activities as compared with the control population. The Rockefeller University Press 1966-03-01 /pmc/articles/PMC2106942/ /pubmed/5960807 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Packer, Lester
Nobel, Park S.
Gross, Elizabeth L.
Mel, Howard C.
FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title_full FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title_fullStr FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title_full_unstemmed FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title_short FRACTIONATION OF SPINACH CHLOROPLASTS BY FLOW SEDIMENTATION-ELECTROPHORESIS
title_sort fractionation of spinach chloroplasts by flow sedimentation-electrophoresis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106942/
https://www.ncbi.nlm.nih.gov/pubmed/5960807
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AT melhowardc fractionationofspinachchloroplastsbyflowsedimentationelectrophoresis

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