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STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1966
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106944/ https://www.ncbi.nlm.nih.gov/pubmed/5335636 |
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author | Bauer, G. Eric Lindall, Arnold W. Dixit, Padmaker K. Lester, Gordon Lazarow, Arnold |
author_facet | Bauer, G. Eric Lindall, Arnold W. Dixit, Padmaker K. Lester, Gordon Lazarow, Arnold |
author_sort | Bauer, G. Eric |
collection | PubMed |
description | Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules. |
format | Text |
id | pubmed-2106944 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1966 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21069442008-05-01 STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue Bauer, G. Eric Lindall, Arnold W. Dixit, Padmaker K. Lester, Gordon Lazarow, Arnold J Cell Biol Article Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules. The Rockefeller University Press 1966-03-01 /pmc/articles/PMC2106944/ /pubmed/5335636 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Bauer, G. Eric Lindall, Arnold W. Dixit, Padmaker K. Lester, Gordon Lazarow, Arnold STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title | STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title_full | STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title_fullStr | STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title_full_unstemmed | STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title_short | STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue |
title_sort | studies on insulin biosynthesis : subcellular distribution of leucine-h(3) radioactivity during incubation of goosefish islet tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106944/ https://www.ncbi.nlm.nih.gov/pubmed/5335636 |
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