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STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue

Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had...

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Detalles Bibliográficos
Autores principales: Bauer, G. Eric, Lindall, Arnold W., Dixit, Padmaker K., Lester, Gordon, Lazarow, Arnold
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106944/
https://www.ncbi.nlm.nih.gov/pubmed/5335636
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author Bauer, G. Eric
Lindall, Arnold W.
Dixit, Padmaker K.
Lester, Gordon
Lazarow, Arnold
author_facet Bauer, G. Eric
Lindall, Arnold W.
Dixit, Padmaker K.
Lester, Gordon
Lazarow, Arnold
author_sort Bauer, G. Eric
collection PubMed
description Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.
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spelling pubmed-21069442008-05-01 STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue Bauer, G. Eric Lindall, Arnold W. Dixit, Padmaker K. Lester, Gordon Lazarow, Arnold J Cell Biol Article Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H(3). The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules. The Rockefeller University Press 1966-03-01 /pmc/articles/PMC2106944/ /pubmed/5335636 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bauer, G. Eric
Lindall, Arnold W.
Dixit, Padmaker K.
Lester, Gordon
Lazarow, Arnold
STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title_full STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title_fullStr STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title_full_unstemmed STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title_short STUDIES ON INSULIN BIOSYNTHESIS : Subcellular Distribution of Leucine-H(3) Radioactivity During Incubation of Goosefish Islet Tissue
title_sort studies on insulin biosynthesis : subcellular distribution of leucine-h(3) radioactivity during incubation of goosefish islet tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106944/
https://www.ncbi.nlm.nih.gov/pubmed/5335636
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