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PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines
On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in...
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1966
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106989/ https://www.ncbi.nlm.nih.gov/pubmed/4165077 |
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author | Chapman, D. Fluck, D. J. |
author_facet | Chapman, D. Fluck, D. J. |
author_sort | Chapman, D. |
collection | PubMed |
description | On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in terms of the large difference in the transition temperature for "melting" of the hydrocarbon chains of unsaturated and saturated phospholipids and is illustrated by means of differential thermal analysis (D.T.A.) curves. These structures have been examined by electron microscopy after negative staining and after reaction with osmium tetroxide. Typical phospholipid lamella structures are seen in the phosphatidylcholines after negative staining, and in the phosphatidyl-ethanolamines after both negative staining and osmium fixation. The distances across these lamellae have been measured. Some preliminary investigations of the nature of the osmium tetroxide reaction with the phosphatidyl-ethanolamines have been made. |
format | Text |
id | pubmed-2106989 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1966 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21069892008-05-01 PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines Chapman, D. Fluck, D. J. J Cell Biol Article On heating pure, fully saturated 2,3-diacyl-DL-phosphatidyl-ethanolamines and 2,3-diacylphosphatidyl-cholines (lecithins) in water to the transition temperature at which large endothermic heat changes occur, they are observed, by light microscopy, to form myelin figures. This result is discussed in terms of the large difference in the transition temperature for "melting" of the hydrocarbon chains of unsaturated and saturated phospholipids and is illustrated by means of differential thermal analysis (D.T.A.) curves. These structures have been examined by electron microscopy after negative staining and after reaction with osmium tetroxide. Typical phospholipid lamella structures are seen in the phosphatidylcholines after negative staining, and in the phosphatidyl-ethanolamines after both negative staining and osmium fixation. The distances across these lamellae have been measured. Some preliminary investigations of the nature of the osmium tetroxide reaction with the phosphatidyl-ethanolamines have been made. The Rockefeller University Press 1966-07-01 /pmc/articles/PMC2106989/ /pubmed/4165077 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Chapman, D. Fluck, D. J. PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title | PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title_full | PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title_fullStr | PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title_full_unstemmed | PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title_short | PHYSICAL STUDIES OF PHOSPHOLIPIDS : III. Electron Microscope Studies of Some Pure Fully Saturated 2,3-Diacyl-DL-Phosphatidyl-Ethanolamines and Phosphatidyl-Cholines |
title_sort | physical studies of phospholipids : iii. electron microscope studies of some pure fully saturated 2,3-diacyl-dl-phosphatidyl-ethanolamines and phosphatidyl-cholines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2106989/ https://www.ncbi.nlm.nih.gov/pubmed/4165077 |
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