ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI

A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate...

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Detalles Bibliográficos
Autores principales: Løvtrup-Rein, H., McEwen, B. S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107004/
https://www.ncbi.nlm.nih.gov/pubmed/5968977
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author Løvtrup-Rein, H.
McEwen, B. S.
author_facet Løvtrup-Rein, H.
McEwen, B. S.
author_sort Løvtrup-Rein, H.
collection PubMed
description A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate. Both the morphology and size of the nuclei remained unchanged. Problems concerning the composition of the isolation media, the use of detergents, as well as those raised by density gradient centrifugation in sucrose, Ficoll, and Dextran are discussed. Some values for the density of each type of brain nuclei are suggested.
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spelling pubmed-21070042008-05-01 ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI Løvtrup-Rein, H. McEwen, B. S. J Cell Biol Article A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate. Both the morphology and size of the nuclei remained unchanged. Problems concerning the composition of the isolation media, the use of detergents, as well as those raised by density gradient centrifugation in sucrose, Ficoll, and Dextran are discussed. Some values for the density of each type of brain nuclei are suggested. The Rockefeller University Press 1966-08-01 /pmc/articles/PMC2107004/ /pubmed/5968977 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Løvtrup-Rein, H.
McEwen, B. S.
ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title_full ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title_fullStr ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title_full_unstemmed ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title_short ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI
title_sort isolation and fractionation of rat brain nuclei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107004/
https://www.ncbi.nlm.nih.gov/pubmed/5968977
work_keys_str_mv AT løvtrupreinh isolationandfractionationofratbrainnuclei
AT mcewenbs isolationandfractionationofratbrainnuclei

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