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THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS

Meiotic chromosomes were isolated from male Oncopeltus fasciatus by dissecting the testes under insect Ringer's solution and spreading the living cells on the Langmuir trough. After being dried by the critical point method, preparations were examined under the electron microscope. Chromosomes a...

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Detalles Bibliográficos
Autores principales: Wolfe, Stephen L., Hewitt, Godfrey M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107030/
https://www.ncbi.nlm.nih.gov/pubmed/5971973
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author Wolfe, Stephen L.
Hewitt, Godfrey M.
author_facet Wolfe, Stephen L.
Hewitt, Godfrey M.
author_sort Wolfe, Stephen L.
collection PubMed
description Meiotic chromosomes were isolated from male Oncopeltus fasciatus by dissecting the testes under insect Ringer's solution and spreading the living cells on the Langmuir trough. After being dried by the critical point method, preparations were examined under the electron microscope. Chromosomes at all stages of prophase prove to be multistranded. A significant increase in the number of parallel 250 A fibers in the chromosomes occurs between zygotene and diakinesis. Parallel folding, rather than true multistrandedness, is interpreted as the mechanism responsible for this observed increase in multistrandedness. It has not been possible to determine whether the multistrandedness observed at leptotene represents true multistrandedness or is the result of parallel folding. Apparent multistrandedness is lost at metaphase when the 250 A fibers of the chromosomes become coiled more tightly. In preparations isolated by these methods, no structures other than the 250 A chromosome fibers are visible in the chromomeres, which appear as regionally coiled or folded areas of the fibers along the arm of the chromosome.
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spelling pubmed-21070302008-05-01 THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS Wolfe, Stephen L. Hewitt, Godfrey M. J Cell Biol Article Meiotic chromosomes were isolated from male Oncopeltus fasciatus by dissecting the testes under insect Ringer's solution and spreading the living cells on the Langmuir trough. After being dried by the critical point method, preparations were examined under the electron microscope. Chromosomes at all stages of prophase prove to be multistranded. A significant increase in the number of parallel 250 A fibers in the chromosomes occurs between zygotene and diakinesis. Parallel folding, rather than true multistrandedness, is interpreted as the mechanism responsible for this observed increase in multistrandedness. It has not been possible to determine whether the multistrandedness observed at leptotene represents true multistrandedness or is the result of parallel folding. Apparent multistrandedness is lost at metaphase when the 250 A fibers of the chromosomes become coiled more tightly. In preparations isolated by these methods, no structures other than the 250 A chromosome fibers are visible in the chromomeres, which appear as regionally coiled or folded areas of the fibers along the arm of the chromosome. The Rockefeller University Press 1966-10-01 /pmc/articles/PMC2107030/ /pubmed/5971973 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wolfe, Stephen L.
Hewitt, Godfrey M.
THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title_full THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title_fullStr THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title_full_unstemmed THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title_short THE STRANDEDNESS OF MEIOTIC CHROMOSOMES FROM ONCOPELTUS
title_sort strandedness of meiotic chromosomes from oncopeltus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107030/
https://www.ncbi.nlm.nih.gov/pubmed/5971973
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