ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically inta...

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Detalles Bibliográficos
Autores principales: Huberman, Joel A., Attardi, Giuseppe
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1966
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107040/
https://www.ncbi.nlm.nih.gov/pubmed/5339564
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author Huberman, Joel A.
Attardi, Giuseppe
author_facet Huberman, Joel A.
Attardi, Giuseppe
author_sort Huberman, Joel A.
collection PubMed
description The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.
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spelling pubmed-21070402008-05-01 ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS Huberman, Joel A. Attardi, Giuseppe J Cell Biol Article The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA. The Rockefeller University Press 1966-10-01 /pmc/articles/PMC2107040/ /pubmed/5339564 Text en Copyright © 1966 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Huberman, Joel A.
Attardi, Giuseppe
ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title_full ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title_fullStr ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title_full_unstemmed ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title_short ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS
title_sort isolation of metaphase chromosomes from hela cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107040/
https://www.ncbi.nlm.nih.gov/pubmed/5339564
work_keys_str_mv AT hubermanjoela isolationofmetaphasechromosomesfromhelacells
AT attardigiuseppe isolationofmetaphasechromosomesfromhelacells

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