THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System

A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore,...

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Detalles Bibliográficos
Autor principal: Rose, George G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107086/
https://www.ncbi.nlm.nih.gov/pubmed/10976203
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author Rose, George G.
author_facet Rose, George G.
author_sort Rose, George G.
collection PubMed
description A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown.
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spelling pubmed-21070862008-05-01 THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System Rose, George G. J Cell Biol Article A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microenvironment between the closely apposed cellophane and cover slip, whereas the other cell products move from chamber to chamber in the circulating nutrient. The resultant environmental conditions directed by the circumfusion systems are highly favorable for maintaining the differentiation of chick embryo tissues over protracted periods; a number of micrographs are shown. The Rockefeller University Press 1967-01-01 /pmc/articles/PMC2107086/ /pubmed/10976203 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rose, George G.
THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title_full THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title_fullStr THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title_full_unstemmed THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title_short THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS : I. Introduction to the Mechanics, Techniques, and Basic Results of a 12-Chamber (In Vitro) Closed Circulatory System
title_sort circumfusion system for multipurpose culture chambers : i. introduction to the mechanics, techniques, and basic results of a 12-chamber (in vitro) closed circulatory system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107086/
https://www.ncbi.nlm.nih.gov/pubmed/10976203
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