ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles

A method is described for preparing, by filtration on Millipore filters, very thin (about 10 µ) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fr...

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Detalles Bibliográficos
Autores principales: Baudhuin, Pierre, Evrard, Philippe, Berthet, Jacques
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107088/
https://www.ncbi.nlm.nih.gov/pubmed/10976209
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author Baudhuin, Pierre
Evrard, Philippe
Berthet, Jacques
author_facet Baudhuin, Pierre
Evrard, Philippe
Berthet, Jacques
author_sort Baudhuin, Pierre
collection PubMed
description A method is described for preparing, by filtration on Millipore filters, very thin (about 10 µ) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations.
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spelling pubmed-21070882008-05-01 ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles Baudhuin, Pierre Evrard, Philippe Berthet, Jacques J Cell Biol Article A method is described for preparing, by filtration on Millipore filters, very thin (about 10 µ) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations. The Rockefeller University Press 1967-01-01 /pmc/articles/PMC2107088/ /pubmed/10976209 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Baudhuin, Pierre
Evrard, Philippe
Berthet, Jacques
ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title_full ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title_fullStr ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title_full_unstemmed ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title_short ELECTRON MICROSCOPIC EXAMINATION OF SUBCELLULAR FRACTIONS : I. The Preparation of Representative Samples from Suspensions of Particles
title_sort electron microscopic examination of subcellular fractions : i. the preparation of representative samples from suspensions of particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107088/
https://www.ncbi.nlm.nih.gov/pubmed/10976209
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