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THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT

The proximal tibial epiphyses of 18–21-day-old fetal rats have been studied by thymidine-(3)H radioautography. The results reveal that the label is incorporated into two types of osteogenic cells: (a) a spindle cell type (A cells) with characteristics generally associated with matrix production, inc...

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Detalles Bibliográficos
Autor principal: Scott, Bronnetta L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107115/
https://www.ncbi.nlm.nih.gov/pubmed/6061712
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author Scott, Bronnetta L.
author_facet Scott, Bronnetta L.
author_sort Scott, Bronnetta L.
collection PubMed
description The proximal tibial epiphyses of 18–21-day-old fetal rats have been studied by thymidine-(3)H radioautography. The results reveal that the label is incorporated into two types of osteogenic cells: (a) a spindle cell type (A cells) with characteristics generally associated with matrix production, including an extensive development of the endoplasmic reticulum and the presence of large intracellular accumulations of a dense, finely granular material, morphologically identifiable as glycogen; and (b) a rounded cell type (B cells) with morphological features similar in degree and kind to those of the developing neutrophilic leukocyte, including an abundance of free ribosomes and mitochondria and a complex Golgi apparatus associated with dense specific granules, morphologically identifiable with primary lysosomes. These results, along with the occurrence of recognizable, labeled, immature, perivascular forms of both of these A and B type cells, lead to the conclusion that the specialization of osteogenic cells into osteoclasts and osteocytes may involve separate pathways of cytodifferentiation.
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spelling pubmed-21071152008-05-01 THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT Scott, Bronnetta L. J Cell Biol Article The proximal tibial epiphyses of 18–21-day-old fetal rats have been studied by thymidine-(3)H radioautography. The results reveal that the label is incorporated into two types of osteogenic cells: (a) a spindle cell type (A cells) with characteristics generally associated with matrix production, including an extensive development of the endoplasmic reticulum and the presence of large intracellular accumulations of a dense, finely granular material, morphologically identifiable as glycogen; and (b) a rounded cell type (B cells) with morphological features similar in degree and kind to those of the developing neutrophilic leukocyte, including an abundance of free ribosomes and mitochondria and a complex Golgi apparatus associated with dense specific granules, morphologically identifiable with primary lysosomes. These results, along with the occurrence of recognizable, labeled, immature, perivascular forms of both of these A and B type cells, lead to the conclusion that the specialization of osteogenic cells into osteoclasts and osteocytes may involve separate pathways of cytodifferentiation. The Rockefeller University Press 1967-10-01 /pmc/articles/PMC2107115/ /pubmed/6061712 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Scott, Bronnetta L.
THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title_full THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title_fullStr THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title_full_unstemmed THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title_short THYMIDINE-(3)H ELECTRON MICROSCOPE RADIOAUTOGRAPHY OF OSTEOGENIC CELLS IN THE FETAL RAT
title_sort thymidine-(3)h electron microscope radioautography of osteogenic cells in the fetal rat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107115/
https://www.ncbi.nlm.nih.gov/pubmed/6061712
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