THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER

Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light...

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Detalles Bibliográficos
Autores principales: Howard, Roger B., Christensen, A. Kent, Gibbs, Frederic A., Pesch, Leroy A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107157/
https://www.ncbi.nlm.nih.gov/pubmed/4294245
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author Howard, Roger B.
Christensen, A. Kent
Gibbs, Frederic A.
Pesch, Leroy A.
author_facet Howard, Roger B.
Christensen, A. Kent
Gibbs, Frederic A.
Pesch, Leroy A.
author_sort Howard, Roger B.
collection PubMed
description Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light and electron microscopy, approximately 75% of the isolated cells retain their structural integrity. The cell suspensions are capable of maintaining endogenous respiration in the presence of 1% albumin for periods of time up to 8 hr. These cell preparations consist almost entirely of parenchymal cells and offer a unique tissue preparation for the study of hepatic metabolism.
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spelling pubmed-21071572008-05-01 THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER Howard, Roger B. Christensen, A. Kent Gibbs, Frederic A. Pesch, Leroy A. J Cell Biol Article Suspensions of isolated parenchymal cells were prepared from rat liver by incubation with collagenase and hyaluronidase followed by mechanical treatment. Utilization of 0.15% collagenase together with 0.15% hyaluronidase yielded adequate numbers of cells for experimental purposes. As shown by light and electron microscopy, approximately 75% of the isolated cells retain their structural integrity. The cell suspensions are capable of maintaining endogenous respiration in the presence of 1% albumin for periods of time up to 8 hr. These cell preparations consist almost entirely of parenchymal cells and offer a unique tissue preparation for the study of hepatic metabolism. The Rockefeller University Press 1967-12-01 /pmc/articles/PMC2107157/ /pubmed/4294245 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Howard, Roger B.
Christensen, A. Kent
Gibbs, Frederic A.
Pesch, Leroy A.
THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title_full THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title_fullStr THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title_full_unstemmed THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title_short THE ENZYMATIC PREPARATION OF ISOLATED INTACT PARENCHYMAL CELLS FROM RAT LIVER
title_sort enzymatic preparation of isolated intact parenchymal cells from rat liver
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107157/
https://www.ncbi.nlm.nih.gov/pubmed/4294245
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