THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers

When cells from lymph nodes or thoracic duct of mice hyperimmunized with protein antigens are cultivated on embryo monolayers in the presence of the antigen, numerous clones of mast cells appear. The histochemical and ultrastructural characteristics of the cells permit their identification as mast c...

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Detalles Bibliográficos
Autores principales: Ginsburg, H., Lagunoff, D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107161/
https://www.ncbi.nlm.nih.gov/pubmed/4863559
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author Ginsburg, H.
Lagunoff, D.
author_facet Ginsburg, H.
Lagunoff, D.
author_sort Ginsburg, H.
collection PubMed
description When cells from lymph nodes or thoracic duct of mice hyperimmunized with protein antigens are cultivated on embryo monolayers in the presence of the antigen, numerous clones of mast cells appear. The histochemical and ultrastructural characteristics of the cells permit their identification as mast cells and distinguish them from the phagocytic histiocytes that usually arise in abundance in similar cultures from unimmunized mouse cells or from immunized mouse cells cultured in the absence of the antigen. Only a few colonies of mast cells appeared in the latter cultures. The basis for the induction of mast cell differentiation is not known.
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spelling pubmed-21071612008-05-01 THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers Ginsburg, H. Lagunoff, D. J Cell Biol Article When cells from lymph nodes or thoracic duct of mice hyperimmunized with protein antigens are cultivated on embryo monolayers in the presence of the antigen, numerous clones of mast cells appear. The histochemical and ultrastructural characteristics of the cells permit their identification as mast cells and distinguish them from the phagocytic histiocytes that usually arise in abundance in similar cultures from unimmunized mouse cells or from immunized mouse cells cultured in the absence of the antigen. Only a few colonies of mast cells appeared in the latter cultures. The basis for the induction of mast cell differentiation is not known. The Rockefeller University Press 1967-12-01 /pmc/articles/PMC2107161/ /pubmed/4863559 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ginsburg, H.
Lagunoff, D.
THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title_full THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title_fullStr THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title_full_unstemmed THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title_short THE IN VITRO DIFFERENTIATION OF MAST CELLS : Cultures of Cells from Immunized Mouse Lymph Nodes and Thoracic Duct Lymph on Fibroblast Monolayers
title_sort in vitro differentiation of mast cells : cultures of cells from immunized mouse lymph nodes and thoracic duct lymph on fibroblast monolayers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107161/
https://www.ncbi.nlm.nih.gov/pubmed/4863559
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