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RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS
Segments cut from growing oat coleoptiles and pea stems were fed glucose-(3)H in presence and absence of the growth hormone indoleacetic acid (IAA). By means of electron microscope radioautography it was demonstrated that new cell wall material is deposited both at the wall surface (apposition) and...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1967
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107166/ https://www.ncbi.nlm.nih.gov/pubmed/6064369 |
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author | Ray, Peter M. |
author_facet | Ray, Peter M. |
author_sort | Ray, Peter M. |
collection | PubMed |
description | Segments cut from growing oat coleoptiles and pea stems were fed glucose-(3)H in presence and absence of the growth hormone indoleacetic acid (IAA). By means of electron microscope radioautography it was demonstrated that new cell wall material is deposited both at the wall surface (apposition) and within the preexisting wall structure (internally). Quantitative profiles for the distribution of incorporation with position through the thickness of the wall were obtained for the thick outer wall of epidermal cells. With both oat coleoptile and pea stem epidermal outer walls, it was found that a larger proportion of the newly synthesized wall material appeared to become incorporated within the wall in the presence of IAA. Extraction experiments on coleoptile tissue showed that activity that had been incorporated into the cell wall interior represented noncellulosic constituents, mainly hemicelluloses, whereas cellulose was deposited largely or entirely by apposition. It seems possible that internal incorporation of hemicelluloses plays a role in the cell wall expansion process that is involved in cell growth. |
format | Text |
id | pubmed-2107166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1967 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21071662008-05-01 RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS Ray, Peter M. J Cell Biol Article Segments cut from growing oat coleoptiles and pea stems were fed glucose-(3)H in presence and absence of the growth hormone indoleacetic acid (IAA). By means of electron microscope radioautography it was demonstrated that new cell wall material is deposited both at the wall surface (apposition) and within the preexisting wall structure (internally). Quantitative profiles for the distribution of incorporation with position through the thickness of the wall were obtained for the thick outer wall of epidermal cells. With both oat coleoptile and pea stem epidermal outer walls, it was found that a larger proportion of the newly synthesized wall material appeared to become incorporated within the wall in the presence of IAA. Extraction experiments on coleoptile tissue showed that activity that had been incorporated into the cell wall interior represented noncellulosic constituents, mainly hemicelluloses, whereas cellulose was deposited largely or entirely by apposition. It seems possible that internal incorporation of hemicelluloses plays a role in the cell wall expansion process that is involved in cell growth. The Rockefeller University Press 1967-12-01 /pmc/articles/PMC2107166/ /pubmed/6064369 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Ray, Peter M. RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title | RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title_full | RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title_fullStr | RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title_full_unstemmed | RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title_short | RADIOAUTOGRAPHIC STUDY OF CELL WALL DEPOSITION IN GROWING PLANT CELLS |
title_sort | radioautographic study of cell wall deposition in growing plant cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107166/ https://www.ncbi.nlm.nih.gov/pubmed/6064369 |
work_keys_str_mv | AT raypeterm radioautographicstudyofcellwalldepositioningrowingplantcells |