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LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases

The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol....

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Detalles Bibliográficos
Autores principales: Bowers, William E., de Duve, Christian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107252/
https://www.ncbi.nlm.nih.gov/pubmed/10976227
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author Bowers, William E.
de Duve, Christian
author_facet Bowers, William E.
de Duve, Christian
author_sort Bowers, William E.
collection PubMed
description The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol. The results confirm and extend the conclusion, derived from previous investigations on normal animals, that the lysosomes of lymphoid tissues form two and possibly three, distinct populations. The evidence indicates that the L(19) population belongs to macrophages and the L(15) group to lymphocytes. The L(30) population appears to be associated with a special type of phagocyte with a high capacity for dextran storage. All three populations seem to contribute to the activities found in soluble form in homogenates of normal lymphoid tissues.
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spelling pubmed-21072522008-05-01 LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases Bowers, William E. de Duve, Christian J Cell Biol Article The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol. The results confirm and extend the conclusion, derived from previous investigations on normal animals, that the lysosomes of lymphoid tissues form two and possibly three, distinct populations. The evidence indicates that the L(19) population belongs to macrophages and the L(15) group to lymphocytes. The L(30) population appears to be associated with a special type of phagocyte with a high capacity for dextran storage. All three populations seem to contribute to the activities found in soluble form in homogenates of normal lymphoid tissues. The Rockefeller University Press 1967-02-01 /pmc/articles/PMC2107252/ /pubmed/10976227 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bowers, William E.
de Duve, Christian
LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title_full LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title_fullStr LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title_full_unstemmed LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title_short LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
title_sort lysosomes in lymphoid tissue : iii. influence of various treatments of the animals on the distribution of acid hydrolases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107252/
https://www.ncbi.nlm.nih.gov/pubmed/10976227
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