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LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases
The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol....
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1967
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107252/ https://www.ncbi.nlm.nih.gov/pubmed/10976227 |
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author | Bowers, William E. de Duve, Christian |
author_facet | Bowers, William E. de Duve, Christian |
author_sort | Bowers, William E. |
collection | PubMed |
description | The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol. The results confirm and extend the conclusion, derived from previous investigations on normal animals, that the lysosomes of lymphoid tissues form two and possibly three, distinct populations. The evidence indicates that the L(19) population belongs to macrophages and the L(15) group to lymphocytes. The L(30) population appears to be associated with a special type of phagocyte with a high capacity for dextran storage. All three populations seem to contribute to the activities found in soluble form in homogenates of normal lymphoid tissues. |
format | Text |
id | pubmed-2107252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1967 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21072522008-05-01 LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases Bowers, William E. de Duve, Christian J Cell Biol Article The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose—0.2 M KCl gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, (14)C-labeled dextran, (51)Cr-labeled erythrocytes, and cortisol. The results confirm and extend the conclusion, derived from previous investigations on normal animals, that the lysosomes of lymphoid tissues form two and possibly three, distinct populations. The evidence indicates that the L(19) population belongs to macrophages and the L(15) group to lymphocytes. The L(30) population appears to be associated with a special type of phagocyte with a high capacity for dextran storage. All three populations seem to contribute to the activities found in soluble form in homogenates of normal lymphoid tissues. The Rockefeller University Press 1967-02-01 /pmc/articles/PMC2107252/ /pubmed/10976227 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Bowers, William E. de Duve, Christian LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title | LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title_full | LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title_fullStr | LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title_full_unstemmed | LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title_short | LYSOSOMES IN LYMPHOID TISSUE : III. Influence of Various Treatments of the Animals on the Distribution of Acid Hydrolases |
title_sort | lysosomes in lymphoid tissue : iii. influence of various treatments of the animals on the distribution of acid hydrolases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107252/ https://www.ncbi.nlm.nih.gov/pubmed/10976227 |
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