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THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus

Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The bindi...

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Detalles Bibliográficos
Autores principales: Borisy, G. G., Taylor, E. W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107308/
https://www.ncbi.nlm.nih.gov/pubmed/6035643
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author Borisy, G. G.
Taylor, E. W.
author_facet Borisy, G. G.
Taylor, E. W.
author_sort Borisy, G. G.
collection PubMed
description Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The binding site protein was shown to have a sedimentation constant of 6S by zone centrifugation. The protein was present in extracts of the isolated mitotic apparatus at a concentration which was several times higher than in whole-egg homogenates. It was extracted from the mitotic apparatus at low ionic strength under conditions which lead to the disappearance of microtubules. No binding could be detected to the 27S protein, previously described by Kane, which is a major protein component of the isolated mitotic apparatus. The properties of the colchicine-bindinG protein, (binding constant, sedimentation constant, Sephadex elution volume) are similar to those obtained with the protein from mammalian cells, sea-urchin sperm tails, and brain tissue, and thus support the conclusion that the protein is a subunit of microtubules.
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spelling pubmed-21073082008-05-01 THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus Borisy, G. G. Taylor, E. W. J Cell Biol Article Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The binding site protein was shown to have a sedimentation constant of 6S by zone centrifugation. The protein was present in extracts of the isolated mitotic apparatus at a concentration which was several times higher than in whole-egg homogenates. It was extracted from the mitotic apparatus at low ionic strength under conditions which lead to the disappearance of microtubules. No binding could be detected to the 27S protein, previously described by Kane, which is a major protein component of the isolated mitotic apparatus. The properties of the colchicine-bindinG protein, (binding constant, sedimentation constant, Sephadex elution volume) are similar to those obtained with the protein from mammalian cells, sea-urchin sperm tails, and brain tissue, and thus support the conclusion that the protein is a subunit of microtubules. The Rockefeller University Press 1967-08-01 /pmc/articles/PMC2107308/ /pubmed/6035643 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Borisy, G. G.
Taylor, E. W.
THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title_full THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title_fullStr THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title_full_unstemmed THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title_short THE MECHANISM OF ACTION OF COLCHICINE : Colchicine Binding to Sea Urchin Eggs and the Mitotic Apparatus
title_sort mechanism of action of colchicine : colchicine binding to sea urchin eggs and the mitotic apparatus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107308/
https://www.ncbi.nlm.nih.gov/pubmed/6035643
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