A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS

Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0°. As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperatu...

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Detalles Bibliográficos
Autor principal: Korn, Edward D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1967
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107326/
https://www.ncbi.nlm.nih.gov/pubmed/6035650
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author Korn, Edward D.
author_facet Korn, Edward D.
author_sort Korn, Edward D.
collection PubMed
description Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0°. As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid.
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spelling pubmed-21073262008-05-01 A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS Korn, Edward D. J Cell Biol Article Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0°. As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid. The Rockefeller University Press 1967-08-01 /pmc/articles/PMC2107326/ /pubmed/6035650 Text en Copyright © 1967 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Korn, Edward D.
A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title_full A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title_fullStr A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title_full_unstemmed A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title_short A CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC STUDY OF THE PRODUCTS OF THE REACTION OF OSMIUM TETROXIDE WITH UNSATURATED LIPIDS
title_sort chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107326/
https://www.ncbi.nlm.nih.gov/pubmed/6035650
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AT kornedwardd chromatographicandspectrophotometricstudyoftheproductsofthereactionofosmiumtetroxidewithunsaturatedlipids

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