SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS

Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatm...

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Detalles Bibliográficos
Autores principales: Scott, N. Steele, Shah, Vinod C., Smillie, Robert M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107466/
https://www.ncbi.nlm.nih.gov/pubmed/5675411
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author Scott, N. Steele
Shah, Vinod C.
Smillie, Robert M.
author_facet Scott, N. Steele
Shah, Vinod C.
Smillie, Robert M.
author_sort Scott, N. Steele
collection PubMed
description Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not account for the incorporation of label. Extraction of DNA from the chloroplasts and subsequent density gradient centrifugation of the DNA in CsCl(2) showed that the incorporation was into chloroplast DNA (ρ = 1.686) of high molecular weight.
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spelling pubmed-21074662008-05-01 SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS Scott, N. Steele Shah, Vinod C. Smillie, Robert M. J Cell Biol Article Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not account for the incorporation of label. Extraction of DNA from the chloroplasts and subsequent density gradient centrifugation of the DNA in CsCl(2) showed that the incorporation was into chloroplast DNA (ρ = 1.686) of high molecular weight. The Rockefeller University Press 1968-07-01 /pmc/articles/PMC2107466/ /pubmed/5675411 Text en Copyright © 1968 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Scott, N. Steele
Shah, Vinod C.
Smillie, Robert M.
SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title_full SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title_fullStr SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title_full_unstemmed SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title_short SYNTHESIS OF CHLOROPLAST DNA IN ISOLATED CHLOROPLASTS
title_sort synthesis of chloroplast dna in isolated chloroplasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107466/
https://www.ncbi.nlm.nih.gov/pubmed/5675411
work_keys_str_mv AT scottnsteele synthesisofchloroplastdnainisolatedchloroplasts
AT shahvinodc synthesisofchloroplastdnainisolatedchloroplasts
AT smillierobertm synthesisofchloroplastdnainisolatedchloroplasts

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