Cargando…

THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy

Cultured carrot explants, stimulated to grow rapidly in a medium containing coconut milk, were labeled with radioactive proline. After an initial period of absorption (8 hr for proline-(3)H; 24 hr for proline-(14)C) the tissue was allowed to grow for a further period of 6 days in a similar medium fr...

Descripción completa

Detalles Bibliográficos
Autores principales: Israel, H. W., Salpeter, M. M., Steward, F. C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1968
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107559/
https://www.ncbi.nlm.nih.gov/pubmed/5699938
_version_ 1782138822869385216
author Israel, H. W.
Salpeter, M. M.
Steward, F. C.
author_facet Israel, H. W.
Salpeter, M. M.
Steward, F. C.
author_sort Israel, H. W.
collection PubMed
description Cultured carrot explants, stimulated to grow rapidly in a medium containing coconut milk, were labeled with radioactive proline. After an initial period of absorption (8 hr for proline-(3)H; 24 hr for proline-(14)C) the tissue was allowed to grow for a further period of 6 days in a similar medium free from the radioactivity. Samples were prepared for electron microscopy and radioautography at the end of the absorption period and also after the further growth. The distribution of the products from the radioactive proline in the cells is shown by high-resolution radioautography and is rendered quantitative for the different regions of the cells. The results show that the combined label, which was present in the form of proline and the hydroxyproline derived from it, was all in the protoplasm, not in the cell walls. Any combined label that appeared to be over the cell walls is shown to be due to scatter from adjacent cytoplasmic sites. Initially the radioactivity was concentrated in nuclei, even more so in nucleoli, but it subsequently appeared throughout the ground cytoplasm and was also concentrated in the plastids. The significance of these observations for the general concept of a plant cell wall protein and for the special problem of growth induction in otherwise quiescent cells is discussed.
format Text
id pubmed-2107559
institution National Center for Biotechnology Information
language English
publishDate 1968
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21075592008-05-01 THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy Israel, H. W. Salpeter, M. M. Steward, F. C. J Cell Biol Article Cultured carrot explants, stimulated to grow rapidly in a medium containing coconut milk, were labeled with radioactive proline. After an initial period of absorption (8 hr for proline-(3)H; 24 hr for proline-(14)C) the tissue was allowed to grow for a further period of 6 days in a similar medium free from the radioactivity. Samples were prepared for electron microscopy and radioautography at the end of the absorption period and also after the further growth. The distribution of the products from the radioactive proline in the cells is shown by high-resolution radioautography and is rendered quantitative for the different regions of the cells. The results show that the combined label, which was present in the form of proline and the hydroxyproline derived from it, was all in the protoplasm, not in the cell walls. Any combined label that appeared to be over the cell walls is shown to be due to scatter from adjacent cytoplasmic sites. Initially the radioactivity was concentrated in nuclei, even more so in nucleoli, but it subsequently appeared throughout the ground cytoplasm and was also concentrated in the plastids. The significance of these observations for the general concept of a plant cell wall protein and for the special problem of growth induction in otherwise quiescent cells is discussed. The Rockefeller University Press 1968-12-01 /pmc/articles/PMC2107559/ /pubmed/5699938 Text en Copyright © 1968 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Israel, H. W.
Salpeter, M. M.
Steward, F. C.
THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title_full THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title_fullStr THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title_full_unstemmed THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title_short THE INCORPORATION OF RADIOACTIVE PROLINE INTO CULTURED CELLS : Interpretations Based On Radioautography and Electron Microscopy
title_sort incorporation of radioactive proline into cultured cells : interpretations based on radioautography and electron microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107559/
https://www.ncbi.nlm.nih.gov/pubmed/5699938
work_keys_str_mv AT israelhw theincorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy
AT salpetermm theincorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy
AT stewardfc theincorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy
AT israelhw incorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy
AT salpetermm incorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy
AT stewardfc incorporationofradioactiveprolineintoculturedcellsinterpretationsbasedonradioautographyandelectronmicroscopy