RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)

Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total...

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Detalles Bibliográficos
Autores principales: Enger, M. D., Tobey, R. A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107561/
https://www.ncbi.nlm.nih.gov/pubmed/5795885
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author Enger, M. D.
Tobey, R. A.
author_facet Enger, M. D.
Tobey, R. A.
author_sort Enger, M. D.
collection PubMed
description Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.
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spelling pubmed-21075612008-05-01 RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1) Enger, M. D. Tobey, R. A. J Cell Biol Article Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis. The Rockefeller University Press 1969-07-01 /pmc/articles/PMC2107561/ /pubmed/5795885 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Enger, M. D.
Tobey, R. A.
RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title_full RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title_fullStr RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title_full_unstemmed RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title_short RNA SYNTHESIS IN CHINESE HAMSTER CELLS : II. Increase in Rate of RNA Synthesis during G(1)
title_sort rna synthesis in chinese hamster cells : ii. increase in rate of rna synthesis during g(1)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107561/
https://www.ncbi.nlm.nih.gov/pubmed/5795885
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