HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties

A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and o...

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Detalles Bibliográficos
Autores principales: Tesar, Joseph T., Koenig, Harold, Hughes, Charles
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107604/
https://www.ncbi.nlm.nih.gov/pubmed/5782447
Descripción
Sumario:A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and of small granules is 220 mµ. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction.

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