HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties

A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and o...

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Detalles Bibliográficos
Autores principales: Tesar, Joseph T., Koenig, Harold, Hughes, Charles
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107604/
https://www.ncbi.nlm.nih.gov/pubmed/5782447
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author Tesar, Joseph T.
Koenig, Harold
Hughes, Charles
author_facet Tesar, Joseph T.
Koenig, Harold
Hughes, Charles
author_sort Tesar, Joseph T.
collection PubMed
description A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and of small granules is 220 mµ. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction.
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spelling pubmed-21076042008-05-01 HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties Tesar, Joseph T. Koenig, Harold Hughes, Charles J Cell Biol Article A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and of small granules is 220 mµ. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction. The Rockefeller University Press 1969-01-01 /pmc/articles/PMC2107604/ /pubmed/5782447 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tesar, Joseph T.
Koenig, Harold
Hughes, Charles
HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title_full HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title_fullStr HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title_full_unstemmed HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title_short HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY : I. Isolation, Ultrastructure, and Some Biochemical Properties
title_sort hormone storage granules in the beef anterior pituitary : i. isolation, ultrastructure, and some biochemical properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107604/
https://www.ncbi.nlm.nih.gov/pubmed/5782447
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AT hughescharles hormonestoragegranulesinthebeefanteriorpituitaryiisolationultrastructureandsomebiochemicalproperties

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