ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS

Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. E...

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Detalles Bibliográficos
Autores principales: Rajalakshmi, Srinivasan, Adams, W. Robert, Handschumacher, Robert E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107756/
https://www.ncbi.nlm.nih.gov/pubmed/5783877
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author Rajalakshmi, Srinivasan
Adams, W. Robert
Handschumacher, Robert E.
author_facet Rajalakshmi, Srinivasan
Adams, W. Robert
Handschumacher, Robert E.
author_sort Rajalakshmi, Srinivasan
collection PubMed
description Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. Electron microscopy indicated large droplets of lipid bounded by a membrane with attached ribosome-like particles. The presence of ribosomes in these structures was established by treatment with deoxycholate and centrifugation. The proportion of 18S and 29S RNA was the same as that found in the ribosomes from normal liver; however, the distribution of radioactivity between the 18S and the 29S RNA after injection of 8-(14)C-adenine was distinctly different. The RNA isolated from these structures contained a higher guanylic acid to cytidylic acid ratio than that found in the microsomes of the normal liver. It is proposed that these low density structures may be those portions of the endoplasmic reticulum in which there exists a defect responsible for the block in the assembly or secretion of plasma lipoprotein.
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spelling pubmed-21077562008-05-01 ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS Rajalakshmi, Srinivasan Adams, W. Robert Handschumacher, Robert E. J Cell Biol Article Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. Electron microscopy indicated large droplets of lipid bounded by a membrane with attached ribosome-like particles. The presence of ribosomes in these structures was established by treatment with deoxycholate and centrifugation. The proportion of 18S and 29S RNA was the same as that found in the ribosomes from normal liver; however, the distribution of radioactivity between the 18S and the 29S RNA after injection of 8-(14)C-adenine was distinctly different. The RNA isolated from these structures contained a higher guanylic acid to cytidylic acid ratio than that found in the microsomes of the normal liver. It is proposed that these low density structures may be those portions of the endoplasmic reticulum in which there exists a defect responsible for the block in the assembly or secretion of plasma lipoprotein. The Rockefeller University Press 1969-05-01 /pmc/articles/PMC2107756/ /pubmed/5783877 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Rajalakshmi, Srinivasan
Adams, W. Robert
Handschumacher, Robert E.
ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title_full ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title_fullStr ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title_full_unstemmed ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title_short ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
title_sort isolation and characterization of low density structures from orotic acid-induced fatty livers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107756/
https://www.ncbi.nlm.nih.gov/pubmed/5783877
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