ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS
Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. E...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1969
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107756/ https://www.ncbi.nlm.nih.gov/pubmed/5783877 |
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author | Rajalakshmi, Srinivasan Adams, W. Robert Handschumacher, Robert E. |
author_facet | Rajalakshmi, Srinivasan Adams, W. Robert Handschumacher, Robert E. |
author_sort | Rajalakshmi, Srinivasan |
collection | PubMed |
description | Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. Electron microscopy indicated large droplets of lipid bounded by a membrane with attached ribosome-like particles. The presence of ribosomes in these structures was established by treatment with deoxycholate and centrifugation. The proportion of 18S and 29S RNA was the same as that found in the ribosomes from normal liver; however, the distribution of radioactivity between the 18S and the 29S RNA after injection of 8-(14)C-adenine was distinctly different. The RNA isolated from these structures contained a higher guanylic acid to cytidylic acid ratio than that found in the microsomes of the normal liver. It is proposed that these low density structures may be those portions of the endoplasmic reticulum in which there exists a defect responsible for the block in the assembly or secretion of plasma lipoprotein. |
format | Text |
id | pubmed-2107756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1969 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21077562008-05-01 ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS Rajalakshmi, Srinivasan Adams, W. Robert Handschumacher, Robert E. J Cell Biol Article Centrifugation of a sucrose homogenate of the livers of female albino rats fed a 1.5% orotic acid diet for 3 wk yielded a pellicle containing low density structures. In morphology and biochemical properties these structures resembled those portions of endoplasmic reticulum which accumulated lipid. Electron microscopy indicated large droplets of lipid bounded by a membrane with attached ribosome-like particles. The presence of ribosomes in these structures was established by treatment with deoxycholate and centrifugation. The proportion of 18S and 29S RNA was the same as that found in the ribosomes from normal liver; however, the distribution of radioactivity between the 18S and the 29S RNA after injection of 8-(14)C-adenine was distinctly different. The RNA isolated from these structures contained a higher guanylic acid to cytidylic acid ratio than that found in the microsomes of the normal liver. It is proposed that these low density structures may be those portions of the endoplasmic reticulum in which there exists a defect responsible for the block in the assembly or secretion of plasma lipoprotein. The Rockefeller University Press 1969-05-01 /pmc/articles/PMC2107756/ /pubmed/5783877 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Rajalakshmi, Srinivasan Adams, W. Robert Handschumacher, Robert E. ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title | ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title_full | ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title_fullStr | ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title_full_unstemmed | ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title_short | ISOLATION AND CHARACTERIZATION OF LOW DENSITY STRUCTURES FROM OROTIC ACID-INDUCED FATTY LIVERS |
title_sort | isolation and characterization of low density structures from orotic acid-induced fatty livers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107756/ https://www.ncbi.nlm.nih.gov/pubmed/5783877 |
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