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ISOLATION OF PLASMA MEMBRANE FRAGMENTS FROM HELA CELLS

A method for isolating plasma membrane fragments from HeLa cells is described. The procedure starts with the preparation of cell membrane "ghosts," obtained by gentle rupture of hypotonically swollen cells, evacuation of most of the cell contents by repeated washing, and isolation of the g...

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Detalles Bibliográficos
Autores principales: Boone, Charles W., Ford, Lincoln E., Bond, Howard E., Stuart, Donald C., Lorenz, Dianne
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107758/
https://www.ncbi.nlm.nih.gov/pubmed/4239370