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EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL

With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was...

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Detalles Bibliográficos
Autor principal: Tanaka, Yasukazu
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107762/
https://www.ncbi.nlm.nih.gov/pubmed/4181966
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author Tanaka, Yasukazu
author_facet Tanaka, Yasukazu
author_sort Tanaka, Yasukazu
collection PubMed
description With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was noted in the area extending 8–10 µ beyond the nonpreferential damage of 4–5 µ in diameter. The opaque material associated with mitochondria possibly represented coagulated protein. The effect involved cristae mitochondriales without severe disarrangement of their structure. The opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam, even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 µ Within the thickness of 2–4 µ of monolayered cells, larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length. A threshold of biologic effectiveness is probably also involved.
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spelling pubmed-21077622008-05-01 EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL Tanaka, Yasukazu J Cell Biol Article With pinacyanol as the supravital stain, a preferential effect on mitochondria of KB cells was achieved by the irradiation with the ruby laser beam. The observation confirmed the results of other workers using janus green B in the same experimental system. The preferential effect on mitochondria was noted in the area extending 8–10 µ beyond the nonpreferential damage of 4–5 µ in diameter. The opaque material associated with mitochondria possibly represented coagulated protein. The effect involved cristae mitochondriales without severe disarrangement of their structure. The opaque material could be interpreted as the result of direct interaction between mitochondria and the laser beam, even though the mitochondria were noted outside of the previously estimated focal spot size of about 3 µ Within the thickness of 2–4 µ of monolayered cells, larger areas of damage can be accounted for by divergence of the beam which is focused by a microscope objective of very short focal length. A threshold of biologic effectiveness is probably also involved. The Rockefeller University Press 1969-05-01 /pmc/articles/PMC2107762/ /pubmed/4181966 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tanaka, Yasukazu
EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title_full EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title_fullStr EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title_full_unstemmed EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title_short EFFECT OF THE RUBY LASER MICROBEAM ON MITOCHONDRIA OF KB CELLS SUPRAVITALLY STAINED BY PINACYANOL
title_sort effect of the ruby laser microbeam on mitochondria of kb cells supravitally stained by pinacyanol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107762/
https://www.ncbi.nlm.nih.gov/pubmed/4181966
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