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LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT
The mitochondrial derivative of the sperm cell of the Gastropod Helix is endowed with a compartment loaded with glycogen (Personne et André, 1964). The aim of this work is to establish whether this mitochondrial glycogen is synthesized in the mitochondrial derivative itself or elsewhere in the cell....
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1970
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107781/ https://www.ncbi.nlm.nih.gov/pubmed/5409457 |
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author | Personne, Paul Anderson, Winston |
author_facet | Personne, Paul Anderson, Winston |
author_sort | Personne, Paul |
collection | PubMed |
description | The mitochondrial derivative of the sperm cell of the Gastropod Helix is endowed with a compartment loaded with glycogen (Personne et André, 1964). The aim of this work is to establish whether this mitochondrial glycogen is synthesized in the mitochondrial derivative itself or elsewhere in the cell. For this purpose, living sperm were first incubated in a medium containing glucose 1-phosphate, then fixed, sectioned, and stained specifically for polysaccharides by the phosphotungstic acid technique or the periodic acid-thiosemicarbazide-silver proteinate technique. Comparison with controls shows that a synthesis of glycogen occurred during the time of incubation. It can be inferred from this result that an amylophosphorylase system controlling the metabolism of polysaccharides is present in the mitochondrial derivative itself. Results obtained with the iodine technique indicate that the original glycogen molecules are elongated during the experiment. It seems probable that the amylophosphorylase system demonstrated here accounts for at least a part of the proteinaceous coat morphologically detectable around each individual glycogen particle. |
format | Text |
id | pubmed-2107781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1970 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21077812008-05-01 LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT Personne, Paul Anderson, Winston J Cell Biol Article The mitochondrial derivative of the sperm cell of the Gastropod Helix is endowed with a compartment loaded with glycogen (Personne et André, 1964). The aim of this work is to establish whether this mitochondrial glycogen is synthesized in the mitochondrial derivative itself or elsewhere in the cell. For this purpose, living sperm were first incubated in a medium containing glucose 1-phosphate, then fixed, sectioned, and stained specifically for polysaccharides by the phosphotungstic acid technique or the periodic acid-thiosemicarbazide-silver proteinate technique. Comparison with controls shows that a synthesis of glycogen occurred during the time of incubation. It can be inferred from this result that an amylophosphorylase system controlling the metabolism of polysaccharides is present in the mitochondrial derivative itself. Results obtained with the iodine technique indicate that the original glycogen molecules are elongated during the experiment. It seems probable that the amylophosphorylase system demonstrated here accounts for at least a part of the proteinaceous coat morphologically detectable around each individual glycogen particle. The Rockefeller University Press 1970-01-01 /pmc/articles/PMC2107781/ /pubmed/5409457 Text en Copyright © 1970 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Personne, Paul Anderson, Winston LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title | LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title_full | LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title_fullStr | LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title_full_unstemmed | LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title_short | LOCALISATION MITOCHONDRIALE D'ENZYMES LIEES AU METABOLISME DU GLYCOGENE DANS LE SPERMATOZOIDE DE L'ESCARGOT |
title_sort | localisation mitochondriale d'enzymes liees au metabolisme du glycogene dans le spermatozoide de l'escargot |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107781/ https://www.ncbi.nlm.nih.gov/pubmed/5409457 |
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