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RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS

Rat thyroid lobes incubated with mannose-(3)H, galactose-(3)H, or leucine-(3)H, were studied by radioautography. With leucine-(3)H and mannose-(3)H, the grain reaction observed in the light microscope is distributed diffusely over the cells at 5 min, with no reaction over the colloid. Later, the gra...

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Detalles Bibliográficos
Autores principales: Whur, P., Herscovics, Annette, Leblond, C. P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1969
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107858/
https://www.ncbi.nlm.nih.gov/pubmed/5344149
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author Whur, P.
Herscovics, Annette
Leblond, C. P.
author_facet Whur, P.
Herscovics, Annette
Leblond, C. P.
author_sort Whur, P.
collection PubMed
description Rat thyroid lobes incubated with mannose-(3)H, galactose-(3)H, or leucine-(3)H, were studied by radioautography. With leucine-(3)H and mannose-(3)H, the grain reaction observed in the light microscope is distributed diffusely over the cells at 5 min, with no reaction over the colloid. Later, the grains are concentrated towards the apex, and colloid reactions begin to appear by 2 hr. With galactose-(3)H, the reaction at 5 min is again restricted to the cells but it consists of clumped grains next to the nucleus. Soon after, grains are concentrated at the cell apex and colloid reactions appear in some follicles as early as 30 min. Puromycin almost totally inhibits incorporation of leucine-(3)H and mannose-(3)H, but has no detectable effect on galactose-(3)H incorporation during the 1st hr. Quantitation of electron microscope radioautographs shows that mannose-(3)H label localizes initially in the rough endoplasmic reticulum, and by 1–2 hr much of this reaction is transferred to the Golgi apparatus. At 3 hr and subsequently, significant reactions are present over apical vesicles and colloid, while the Golgi reaction declines. Label associated with galactose-(3)H localizes initially in the Golgi apparatus and rapidly transfers to the apical vesicles, and then to the colloid. These findings indicate that mannose incorporation into thyroglobulin precursors occurs within the rough endoplasmic reticulum; these precursors then migrate to the Golgi apparatus, where galactose incorporation takes place. The glycoprotein thus formed migrates via the apical vesicles to the colloid.
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spelling pubmed-21078582008-05-01 RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS Whur, P. Herscovics, Annette Leblond, C. P. J Cell Biol Article Rat thyroid lobes incubated with mannose-(3)H, galactose-(3)H, or leucine-(3)H, were studied by radioautography. With leucine-(3)H and mannose-(3)H, the grain reaction observed in the light microscope is distributed diffusely over the cells at 5 min, with no reaction over the colloid. Later, the grains are concentrated towards the apex, and colloid reactions begin to appear by 2 hr. With galactose-(3)H, the reaction at 5 min is again restricted to the cells but it consists of clumped grains next to the nucleus. Soon after, grains are concentrated at the cell apex and colloid reactions appear in some follicles as early as 30 min. Puromycin almost totally inhibits incorporation of leucine-(3)H and mannose-(3)H, but has no detectable effect on galactose-(3)H incorporation during the 1st hr. Quantitation of electron microscope radioautographs shows that mannose-(3)H label localizes initially in the rough endoplasmic reticulum, and by 1–2 hr much of this reaction is transferred to the Golgi apparatus. At 3 hr and subsequently, significant reactions are present over apical vesicles and colloid, while the Golgi reaction declines. Label associated with galactose-(3)H localizes initially in the Golgi apparatus and rapidly transfers to the apical vesicles, and then to the colloid. These findings indicate that mannose incorporation into thyroglobulin precursors occurs within the rough endoplasmic reticulum; these precursors then migrate to the Golgi apparatus, where galactose incorporation takes place. The glycoprotein thus formed migrates via the apical vesicles to the colloid. The Rockefeller University Press 1969-11-01 /pmc/articles/PMC2107858/ /pubmed/5344149 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Whur, P.
Herscovics, Annette
Leblond, C. P.
RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title_full RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title_fullStr RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title_full_unstemmed RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title_short RADIOAUTOGRAPHIC VISUALIZATION OF THE INCORPORATION OF GALACTOSE-(3)H AND MANNOSE-(3)H BY RAT THYROIDS IN VITRO IN RELATION TO THE STAGES OF THYROGLOBULIN SYNTHESIS
title_sort radioautographic visualization of the incorporation of galactose-(3)h and mannose-(3)h by rat thyroids in vitro in relation to the stages of thyroglobulin synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107858/
https://www.ncbi.nlm.nih.gov/pubmed/5344149
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