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THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE
The production of Golgi complexes was investigated in Amoeba proteus by introducing a nucleus into cells that had been enucleated for 5 days. Golgi complexes were not detected in 5 day enucleates, nor were they observed in amebae fixed 15 min after renucleation. Samples taken at longer intervals aft...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1969
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107869/ https://www.ncbi.nlm.nih.gov/pubmed/5344148 |
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author | Flickinger, Charles J. |
author_facet | Flickinger, Charles J. |
author_sort | Flickinger, Charles J. |
collection | PubMed |
description | The production of Golgi complexes was investigated in Amoeba proteus by introducing a nucleus into cells that had been enucleated for 5 days. Golgi complexes were not detected in 5 day enucleates, nor were they observed in amebae fixed 15 min after renucleation. Samples taken at longer intervals after the introduction of a nucleus exhibited an increase in the size and abundance of Golgi complexes. Small curved smooth cisternae, some of which were aligned in parallel to form small Golgi complexes, were observed 30 min after the operation. Aggregations of small Golgi complexes increased in number in amebae fixed 1 to 6 hr after renucleation. Golgi complexes of normal size were present 6 hr after the operation and became more abundant in samples fixed 12 hr, and 1, 2, and 3 days after renucleation. The possible participation of the granular endoplasmic reticulum in the development of Golgi complexes was suggested by two observations. First, the Golgi complexes in renucleates contained a dense material similar to the content of the endoplasmic reticulum in enucleates and early renucleates. Second, examples of continuity between the endoplasmic reticulum and Golgi cisternae were present in renucleates. The possibility that Golgi complexes can be produced in the absence of preexisting Golgi complexes is discussed. |
format | Text |
id | pubmed-2107869 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1969 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21078692008-05-01 THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE Flickinger, Charles J. J Cell Biol Article The production of Golgi complexes was investigated in Amoeba proteus by introducing a nucleus into cells that had been enucleated for 5 days. Golgi complexes were not detected in 5 day enucleates, nor were they observed in amebae fixed 15 min after renucleation. Samples taken at longer intervals after the introduction of a nucleus exhibited an increase in the size and abundance of Golgi complexes. Small curved smooth cisternae, some of which were aligned in parallel to form small Golgi complexes, were observed 30 min after the operation. Aggregations of small Golgi complexes increased in number in amebae fixed 1 to 6 hr after renucleation. Golgi complexes of normal size were present 6 hr after the operation and became more abundant in samples fixed 12 hr, and 1, 2, and 3 days after renucleation. The possible participation of the granular endoplasmic reticulum in the development of Golgi complexes was suggested by two observations. First, the Golgi complexes in renucleates contained a dense material similar to the content of the endoplasmic reticulum in enucleates and early renucleates. Second, examples of continuity between the endoplasmic reticulum and Golgi cisternae were present in renucleates. The possibility that Golgi complexes can be produced in the absence of preexisting Golgi complexes is discussed. The Rockefeller University Press 1969-11-01 /pmc/articles/PMC2107869/ /pubmed/5344148 Text en Copyright © 1969 by The Rockefeller University Press. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Flickinger, Charles J. THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title | THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title_full | THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title_fullStr | THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title_full_unstemmed | THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title_short | THE DEVELOPMENT OF GOLGI COMPLEXES AND THEIR DEPENDENCE UPON THE NUCLEUS IN AMEBAE |
title_sort | development of golgi complexes and their dependence upon the nucleus in amebae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107869/ https://www.ncbi.nlm.nih.gov/pubmed/5344148 |
work_keys_str_mv | AT flickingercharlesj thedevelopmentofgolgicomplexesandtheirdependenceuponthenucleusinamebae AT flickingercharlesj developmentofgolgicomplexesandtheirdependenceuponthenucleusinamebae |