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LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources
Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose—0.02 M KCl gradients. Zonal fractions were analyzed f...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1970
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107900/ https://www.ncbi.nlm.nih.gov/pubmed/4327573 |
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author | Canonico, Peter G. Bird, John W. C. |
author_facet | Canonico, Peter G. Bird, John W. C. |
author_sort | Canonico, Peter G. |
collection | PubMed |
description | Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose—0.02 M KCl gradients. Zonal fractions were analyzed for protein, RNA, cytochrome oxidase, and up to six acid hydrolases. The results indicate the presence of two groups of lysosome-like particles. One group contributes approximately 95% of the cathepsin D and acid phosphatase activity and 75% of the acid ribonuclease, β-glucuronidase, and arylsulfatase activity in muscle. It is characterized by a modal equilibrium density of 1.18 that is decreased by starvation, but is not shifted by dextran-500 or Triton WR-1339. The second group has a higher proportion of acid ribonuclease, β-glucuronidase, and arylsulftase; the equilibrium density can be shifted by dextran-500 and Triton WR-1339. It is suggested that this group of lysosomes is derived from macrophages and other connective tissue cells, whereas the former group represents lysosome-like particles from muscle cells. |
format | Text |
id | pubmed-2107900 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1970 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21079002008-05-01 LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources Canonico, Peter G. Bird, John W. C. J Cell Biol Article Postnuclear supernates from homogenates of skeletal muscle from rats subjected to starvation, injections of Triton WR-1339, dextran-500, and dextran + corticosterone were fractionated by means of rate and isopycnic zonal centrifugation in sucrose—0.02 M KCl gradients. Zonal fractions were analyzed for protein, RNA, cytochrome oxidase, and up to six acid hydrolases. The results indicate the presence of two groups of lysosome-like particles. One group contributes approximately 95% of the cathepsin D and acid phosphatase activity and 75% of the acid ribonuclease, β-glucuronidase, and arylsulfatase activity in muscle. It is characterized by a modal equilibrium density of 1.18 that is decreased by starvation, but is not shifted by dextran-500 or Triton WR-1339. The second group has a higher proportion of acid ribonuclease, β-glucuronidase, and arylsulftase; the equilibrium density can be shifted by dextran-500 and Triton WR-1339. It is suggested that this group of lysosomes is derived from macrophages and other connective tissue cells, whereas the former group represents lysosome-like particles from muscle cells. The Rockefeller University Press 1970-05-01 /pmc/articles/PMC2107900/ /pubmed/4327573 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Canonico, Peter G. Bird, John W. C. LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title | LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title_full | LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title_fullStr | LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title_full_unstemmed | LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title_short | LYSOSOMES IN SKELETAL MUSCLE TISSUE : Zonal Centrifugation Evidence for Multiple Cellular Sources |
title_sort | lysosomes in skeletal muscle tissue : zonal centrifugation evidence for multiple cellular sources |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107900/ https://www.ncbi.nlm.nih.gov/pubmed/4327573 |
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