MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker

The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose th...

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Detalles Bibliográficos
Autores principales: da Silva, Pedro Pinto, Branton, Daniel
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107921/
https://www.ncbi.nlm.nih.gov/pubmed/4918216
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author da Silva, Pedro Pinto
Branton, Daniel
author_facet da Silva, Pedro Pinto
Branton, Daniel
author_sort da Silva, Pedro Pinto
collection PubMed
description The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching.
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spelling pubmed-21079212008-05-01 MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker da Silva, Pedro Pinto Branton, Daniel J Cell Biol Article The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching. The Rockefeller University Press 1970-06-01 /pmc/articles/PMC2107921/ /pubmed/4918216 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
da Silva, Pedro Pinto
Branton, Daniel
MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title_full MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title_fullStr MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title_full_unstemmed MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title_short MEMBRANE SPLITTING IN FREEZE-ETCHING : Covalently Bound Ferritin as a Membrane Marker
title_sort membrane splitting in freeze-etching : covalently bound ferritin as a membrane marker
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107921/
https://www.ncbi.nlm.nih.gov/pubmed/4918216
work_keys_str_mv AT dasilvapedropinto membranesplittinginfreezeetchingcovalentlyboundferritinasamembranemarker
AT brantondaniel membranesplittinginfreezeetchingcovalentlyboundferritinasamembranemarker

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