ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES

A method of isolating the thick luminal membrane from homogenates of bladder epithelium is described, which entails pretreatment of the epithelium with fluorescein mercuric acetate and centrifugation of the homogenate on sucrose density gradients. A hexagonal array of hexamers is illustrated by nega...

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Detalles Bibliográficos
Autores principales: Hicks, R. M., Ketterer, B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107928/
https://www.ncbi.nlm.nih.gov/pubmed/5459940
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author Hicks, R. M.
Ketterer, B.
author_facet Hicks, R. M.
Ketterer, B.
author_sort Hicks, R. M.
collection PubMed
description A method of isolating the thick luminal membrane from homogenates of bladder epithelium is described, which entails pretreatment of the epithelium with fluorescein mercuric acetate and centrifugation of the homogenate on sucrose density gradients. A hexagonal array of hexamers is illustrated by negative contrast staining in whole mounts of the isolated thick membrane. Subunits are also shown in tangential sections of this thick membrane, in fixed, embedded bladder epithelium. The significance of the subunits is discussed in the context of membrane structure and permeability.
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spelling pubmed-21079282008-05-01 ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES Hicks, R. M. Ketterer, B. J Cell Biol Article A method of isolating the thick luminal membrane from homogenates of bladder epithelium is described, which entails pretreatment of the epithelium with fluorescein mercuric acetate and centrifugation of the homogenate on sucrose density gradients. A hexagonal array of hexamers is illustrated by negative contrast staining in whole mounts of the isolated thick membrane. Subunits are also shown in tangential sections of this thick membrane, in fixed, embedded bladder epithelium. The significance of the subunits is discussed in the context of membrane structure and permeability. The Rockefeller University Press 1970-06-01 /pmc/articles/PMC2107928/ /pubmed/5459940 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Hicks, R. M.
Ketterer, B.
ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title_full ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title_fullStr ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title_full_unstemmed ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title_short ISOLATION OF THE PLASMA MEMBRANE OF THE LUMINAL SURFACE OF RAT BLADDER EPITHELIUM, AND THE OCCURRENCE OF A HEXAGONAL LATTICE OF SUBUNITS BOTH IN NEGATIVELY STAINED WHOLE MOUNTS AND IN SECTIONED MEMBRANES
title_sort isolation of the plasma membrane of the luminal surface of rat bladder epithelium, and the occurrence of a hexagonal lattice of subunits both in negatively stained whole mounts and in sectioned membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107928/
https://www.ncbi.nlm.nih.gov/pubmed/5459940
work_keys_str_mv AT hicksrm isolationoftheplasmamembraneoftheluminalsurfaceofratbladderepitheliumandtheoccurrenceofahexagonallatticeofsubunitsbothinnegativelystainedwholemountsandinsectionedmembranes
AT kettererb isolationoftheplasmamembraneoftheluminalsurfaceofratbladderepitheliumandtheoccurrenceofahexagonallatticeofsubunitsbothinnegativelystainedwholemountsandinsectionedmembranes

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