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MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B

Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form β-galactosidase in response to isopropylthiogalactoside,...

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Detalles Bibliográficos
Autores principales: Weinbaum, George, Fischman, Donald A., Okuda, Shinichi
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107932/
https://www.ncbi.nlm.nih.gov/pubmed/4918213
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author Weinbaum, George
Fischman, Donald A.
Okuda, Shinichi
author_facet Weinbaum, George
Fischman, Donald A.
Okuda, Shinichi
author_sort Weinbaum, George
collection PubMed
description Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form β-galactosidase in response to isopropylthiogalactoside, or insensitivity to actinomycin D as compared to the normal cell form. The filamentous cells showed a marked decrease in the ability to incorporate N-acetylglucosamine-UL-(14)C into a phenol-soluble glycoprotein fraction relative to the normal cell form or relative to strain E-26 of E. coli grown in the filament-inducing medium. The filaments yielded an envelope-specific phenol-soluble protein fraction markedly reduced in or lacking three proteins as determined by acrylamide gel electrophoresis. Amino acid analysis, and chemical and enzymatic treatments of the envelope-specific phenol-soluble proteins showed striking differences between the fractions obtained from normal and filamentous cells. Electron microscope studies of divalent cation-induced aggregates of the envelope proteins showed different aggregation patterns dependent upon the cell form yielding the protein fraction.
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spelling pubmed-21079322008-05-01 MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B Weinbaum, George Fischman, Donald A. Okuda, Shinichi J Cell Biol Article Nutritionally induced filamentous cell forms of Escherichia coli B were examined for their morphological and biochemical lesions. The filamentous forms showed no significant alteration in total DNA concentration, RNA synthesis, ability to form β-galactosidase in response to isopropylthiogalactoside, or insensitivity to actinomycin D as compared to the normal cell form. The filamentous cells showed a marked decrease in the ability to incorporate N-acetylglucosamine-UL-(14)C into a phenol-soluble glycoprotein fraction relative to the normal cell form or relative to strain E-26 of E. coli grown in the filament-inducing medium. The filaments yielded an envelope-specific phenol-soluble protein fraction markedly reduced in or lacking three proteins as determined by acrylamide gel electrophoresis. Amino acid analysis, and chemical and enzymatic treatments of the envelope-specific phenol-soluble proteins showed striking differences between the fractions obtained from normal and filamentous cells. Electron microscope studies of divalent cation-induced aggregates of the envelope proteins showed different aggregation patterns dependent upon the cell form yielding the protein fraction. The Rockefeller University Press 1970-06-01 /pmc/articles/PMC2107932/ /pubmed/4918213 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Weinbaum, George
Fischman, Donald A.
Okuda, Shinichi
MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title_full MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title_fullStr MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title_full_unstemmed MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title_short MEMBRANE MODIFICATIONS IN NUTRITIONALLY INDUCED FILAMENTOUS ESCHERICHIA COLI B
title_sort membrane modifications in nutritionally induced filamentous escherichia coli b
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107932/
https://www.ncbi.nlm.nih.gov/pubmed/4918213
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