ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE

The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly al...

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Detalles Bibliográficos
Autor principal: Bryan, Joseph
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107933/
https://www.ncbi.nlm.nih.gov/pubmed/5459944
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author Bryan, Joseph
author_facet Bryan, Joseph
author_sort Bryan, Joseph
collection PubMed
description The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.
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spelling pubmed-21079332008-05-01 ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE Bryan, Joseph J Cell Biol Article The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed. The Rockefeller University Press 1970-06-01 /pmc/articles/PMC2107933/ /pubmed/5459944 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bryan, Joseph
ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title_full ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title_fullStr ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title_full_unstemmed ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title_short ON THE RECONSTITUTION OF THE CRYSTALLINE COMPONENTS OF THE SEA URCHIN FERTILIZATION MEMBRANE
title_sort on the reconstitution of the crystalline components of the sea urchin fertilization membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107933/
https://www.ncbi.nlm.nih.gov/pubmed/5459944
work_keys_str_mv AT bryanjoseph onthereconstitutionofthecrystallinecomponentsoftheseaurchinfertilizationmembrane

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