CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY

It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in the...

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Detalles Bibliográficos
Autores principales: Shelton, Emma, Kuff, Edward L., Maxwell, Elizabeth S., Harrington, J. T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107991/
https://www.ncbi.nlm.nih.gov/pubmed/4917983
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author Shelton, Emma
Kuff, Edward L.
Maxwell, Elizabeth S.
Harrington, J. T.
author_facet Shelton, Emma
Kuff, Edward L.
Maxwell, Elizabeth S.
Harrington, J. T.
author_sort Shelton, Emma
collection PubMed
description It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5–7S and 18–20S. A rectangular particle, 100 x 145 A in size, sediments at 19S and coincides with the second peak of transferase I activity. The possibility that the rectangular particle may be a "carrier" particle associated with transferase I is discussed.
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spelling pubmed-21079912008-05-01 CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY Shelton, Emma Kuff, Edward L. Maxwell, Elizabeth S. Harrington, J. T. J Cell Biol Article It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5–7S and 18–20S. A rectangular particle, 100 x 145 A in size, sediments at 19S and coincides with the second peak of transferase I activity. The possibility that the rectangular particle may be a "carrier" particle associated with transferase I is discussed. The Rockefeller University Press 1970-04-01 /pmc/articles/PMC2107991/ /pubmed/4917983 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Shelton, Emma
Kuff, Edward L.
Maxwell, Elizabeth S.
Harrington, J. T.
CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title_full CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title_fullStr CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title_full_unstemmed CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title_short CYTOPLASMIC PARTICLES AND AMINOACYL TRANSFERASE I ACTIVITY
title_sort cytoplasmic particles and aminoacyl transferase i activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2107991/
https://www.ncbi.nlm.nih.gov/pubmed/4917983
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AT harringtonjt cytoplasmicparticlesandaminoacyltransferaseiactivity

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