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NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE

The round nucleoli of chick embryo myoblasts, when grown in a culture medium devoid of arginine, unravel in several days into 5–20 µ long, beaded strands termed nucleolar necklaces (NN). Addition of arginine reverses this change. The NN contain protein, RNA, and traces of DNA as determined cytochemi...

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Detalles Bibliográficos
Autores principales: Granick, S., Granick, Donna
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108043/
https://www.ncbi.nlm.nih.gov/pubmed/4108477
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author Granick, S.
Granick, Donna
author_facet Granick, S.
Granick, Donna
author_sort Granick, S.
collection PubMed
description The round nucleoli of chick embryo myoblasts, when grown in a culture medium devoid of arginine, unravel in several days into 5–20 µ long, beaded strands termed nucleolar necklaces (NN). Addition of arginine reverses this change. The NN contain protein, RNA, and traces of DNA as determined cytochemically by enzyme digestion and by acridine-orange fluorescent staining. When a cell containing the beaded strand is treated with agents, such as actinomycin D, that prevent rRNA polymerase action, the strand collapses and condenses into a small dense nucleolus with segregated regions of ribonucleoprotein (RNP) and deoxyribonucleoprotein (DNP). The properties of the NN appear to resemble those of the nucleolar necklaces of amphibian oocytes. Cycloheximide or puromycin inhibition of general protein synthesis does not lead to NN formation. We suggest that NN formation during arginine starvation may be a result of a singular depletion of some rapidly turning over, arginine-rich proteins that normally attach to ribosomal RNA precursor molecules during their synthesis in the processing towards maturation of the ribosomes.
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spelling pubmed-21080432008-05-01 NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE Granick, S. Granick, Donna J Cell Biol Article The round nucleoli of chick embryo myoblasts, when grown in a culture medium devoid of arginine, unravel in several days into 5–20 µ long, beaded strands termed nucleolar necklaces (NN). Addition of arginine reverses this change. The NN contain protein, RNA, and traces of DNA as determined cytochemically by enzyme digestion and by acridine-orange fluorescent staining. When a cell containing the beaded strand is treated with agents, such as actinomycin D, that prevent rRNA polymerase action, the strand collapses and condenses into a small dense nucleolus with segregated regions of ribonucleoprotein (RNP) and deoxyribonucleoprotein (DNP). The properties of the NN appear to resemble those of the nucleolar necklaces of amphibian oocytes. Cycloheximide or puromycin inhibition of general protein synthesis does not lead to NN formation. We suggest that NN formation during arginine starvation may be a result of a singular depletion of some rapidly turning over, arginine-rich proteins that normally attach to ribosomal RNA precursor molecules during their synthesis in the processing towards maturation of the ribosomes. The Rockefeller University Press 1971-12-01 /pmc/articles/PMC2108043/ /pubmed/4108477 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Granick, S.
Granick, Donna
NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title_full NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title_fullStr NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title_full_unstemmed NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title_short NUCLEOLAR NECKLACES IN CHICK EMBRYO MYOBLASTS FORMED BY LACK OF ARGININE
title_sort nucleolar necklaces in chick embryo myoblasts formed by lack of arginine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108043/
https://www.ncbi.nlm.nih.gov/pubmed/4108477
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