PURIFICATION OF INTACT MICROTUBULES FROM BRAIN

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay...

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Detalles Bibliográficos
Autores principales: Kirkpatrick, Joel B., Hyams, Lyon, Thomas, Virginia L., Howley, Peter M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1970
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108086/
https://www.ncbi.nlm.nih.gov/pubmed/19866738
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author Kirkpatrick, Joel B.
Hyams, Lyon
Thomas, Virginia L.
Howley, Peter M.
author_facet Kirkpatrick, Joel B.
Hyams, Lyon
Thomas, Virginia L.
Howley, Peter M.
author_sort Kirkpatrick, Joel B.
collection PubMed
description Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.
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spelling pubmed-21080862008-05-01 PURIFICATION OF INTACT MICROTUBULES FROM BRAIN Kirkpatrick, Joel B. Hyams, Lyon Thomas, Virginia L. Howley, Peter M. J Cell Biol Article Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system. The Rockefeller University Press 1970-11-01 /pmc/articles/PMC2108086/ /pubmed/19866738 Text en Copyright © 1970 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kirkpatrick, Joel B.
Hyams, Lyon
Thomas, Virginia L.
Howley, Peter M.
PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title_full PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title_fullStr PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title_full_unstemmed PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title_short PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
title_sort purification of intact microtubules from brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108086/
https://www.ncbi.nlm.nih.gov/pubmed/19866738
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