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PURIFICATION OF INTACT MICROTUBULES FROM BRAIN
Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic Ph, and low temperature. A practical, negative stain, electron microscopic assay...
Autores principales: | Kirkpatrick, Joel B., Hyams, Lyon, Thomas, Virginia L., Howley, Peter M. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1970
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108086/ https://www.ncbi.nlm.nih.gov/pubmed/19866738 |
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