THE M BAND : Studies with Fluorescent Antibody Staining

The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 µ. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence...

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Detalles Bibliográficos
Autores principales: Kundrat, Ellen, Pepe, Frank A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108176/
https://www.ncbi.nlm.nih.gov/pubmed/4101522
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author Kundrat, Ellen
Pepe, Frank A.
author_facet Kundrat, Ellen
Pepe, Frank A.
author_sort Kundrat, Ellen
collection PubMed
description The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 µ. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.
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spelling pubmed-21081762008-05-01 THE M BAND : Studies with Fluorescent Antibody Staining Kundrat, Ellen Pepe, Frank A. J Cell Biol Article The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 µ. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure. The Rockefeller University Press 1971-02-01 /pmc/articles/PMC2108176/ /pubmed/4101522 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kundrat, Ellen
Pepe, Frank A.
THE M BAND : Studies with Fluorescent Antibody Staining
title THE M BAND : Studies with Fluorescent Antibody Staining
title_full THE M BAND : Studies with Fluorescent Antibody Staining
title_fullStr THE M BAND : Studies with Fluorescent Antibody Staining
title_full_unstemmed THE M BAND : Studies with Fluorescent Antibody Staining
title_short THE M BAND : Studies with Fluorescent Antibody Staining
title_sort m band : studies with fluorescent antibody staining
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108176/
https://www.ncbi.nlm.nih.gov/pubmed/4101522
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