A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization

A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypepti...

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Detalles Bibliográficos
Autores principales: Wood, David D., Luck, David J. L.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108245/
https://www.ncbi.nlm.nih.gov/pubmed/4329522
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author Wood, David D.
Luck, David J. L.
author_facet Wood, David D.
Luck, David J. L.
author_sort Wood, David D.
collection PubMed
description A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.
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spelling pubmed-21082452008-05-01 A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization Wood, David D. Luck, David J. L. J Cell Biol Article A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth. The Rockefeller University Press 1971-10-01 /pmc/articles/PMC2108245/ /pubmed/4329522 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wood, David D.
Luck, David J. L.
A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title_full A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title_fullStr A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title_full_unstemmed A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title_short A PARACRYSTALLINE INCLUSION IN NEUROSPORA CRASSA : Induction by Ethidium and Acridine, Isolation, and Characterization
title_sort paracrystalline inclusion in neurospora crassa : induction by ethidium and acridine, isolation, and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108245/
https://www.ncbi.nlm.nih.gov/pubmed/4329522
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