LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE

Methods for the mass isolation of diverse organs from small animals are described. They involve novel devices: a mechanical dissecting system, a centrifugal agitator for the separation of fibrillar from globular particles, and a settling chamber for the fractionation at unit gravity of particles wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Zweidler, Alfred, Cohen, Leonard H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108257/
https://www.ncbi.nlm.nih.gov/pubmed/5000070
_version_ 1782139001233211392
author Zweidler, Alfred
Cohen, Leonard H.
author_facet Zweidler, Alfred
Cohen, Leonard H.
author_sort Zweidler, Alfred
collection PubMed
description Methods for the mass isolation of diverse organs from small animals are described. They involve novel devices: a mechanical dissecting system, a centrifugal agitator for the separation of fibrillar from globular particles, and a settling chamber for the fractionation at unit gravity of particles with sedimentation velocities above the useful range for centrifugation. The application of these methods to the isolation of polytene and nonpolytene nuclei from Drosophila melanogaster larvae is described.
format Text
id pubmed-2108257
institution National Center for Biotechnology Information
language English
publishDate 1971
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21082572008-05-01 LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE Zweidler, Alfred Cohen, Leonard H. J Cell Biol Article Methods for the mass isolation of diverse organs from small animals are described. They involve novel devices: a mechanical dissecting system, a centrifugal agitator for the separation of fibrillar from globular particles, and a settling chamber for the fractionation at unit gravity of particles with sedimentation velocities above the useful range for centrifugation. The application of these methods to the isolation of polytene and nonpolytene nuclei from Drosophila melanogaster larvae is described. The Rockefeller University Press 1971-10-01 /pmc/articles/PMC2108257/ /pubmed/5000070 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Zweidler, Alfred
Cohen, Leonard H.
LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title_full LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title_fullStr LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title_full_unstemmed LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title_short LARGE-SCALE ISOLATION AND FRACTIONATION OF ORGANS OF DROSOPHILA MELANOGASTER LARVAE
title_sort large-scale isolation and fractionation of organs of drosophila melanogaster larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108257/
https://www.ncbi.nlm.nih.gov/pubmed/5000070
work_keys_str_mv AT zweidleralfred largescaleisolationandfractionationoforgansofdrosophilamelanogasterlarvae
AT cohenleonardh largescaleisolationandfractionationoforgansofdrosophilamelanogasterlarvae

Ejemplares similares