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DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules

The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they...

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Detalles Bibliográficos
Autores principales: Nichols, Barbara A., Bainton, Dorothy Ford, Farquhar, Marilyn G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108281/
https://www.ncbi.nlm.nih.gov/pubmed/4107019
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author Nichols, Barbara A.
Bainton, Dorothy Ford
Farquhar, Marilyn G.
author_facet Nichols, Barbara A.
Bainton, Dorothy Ford
Farquhar, Marilyn G.
author_sort Nichols, Barbara A.
collection PubMed
description The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they function as macrophages. Cells were examined from all three phases. It was found that: azurophil granules originate in the Golgi complex of the developing monocyte of bone marrow and blood, and ultimately fuse with phagosomes during phagocytosis upon arrival of monocytes in the tissues. They contain lysosomal enzymes in all species studied and peroxidase in the guinea pig and human. These enzymes are produced by the same pathway as other secretory products (i.e., they are segregated in the rough ER and packaged into granules in the Golgi complex). The findings demonstrate that the azurophil granules of monocytes are primary lysosomes or storage granules comparable to the azurophils of polymorphonuclear leukocytes and the specific granules of eosinophils. Macrophages from peritoneal exudates (72–96 hr after endotoxin injection) contain large quantities of lysosomal enzymes throughout the secretory apparatus (rough ER and Golgi complex), in digestive vacuoles, and in numerous coated vesicles; however, they lack forming or mature azurophil granules. Hence it appears that the monocyte produces two types of primary lysosomes during different phases of its life cycle—azurophil granules made by developing monocytes in bone marrow or blood, and coated vesicles made by macrophages in tissues and body cavities.
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spelling pubmed-21082812008-05-01 DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules Nichols, Barbara A. Bainton, Dorothy Ford Farquhar, Marilyn G. J Cell Biol Article The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they function as macrophages. Cells were examined from all three phases. It was found that: azurophil granules originate in the Golgi complex of the developing monocyte of bone marrow and blood, and ultimately fuse with phagosomes during phagocytosis upon arrival of monocytes in the tissues. They contain lysosomal enzymes in all species studied and peroxidase in the guinea pig and human. These enzymes are produced by the same pathway as other secretory products (i.e., they are segregated in the rough ER and packaged into granules in the Golgi complex). The findings demonstrate that the azurophil granules of monocytes are primary lysosomes or storage granules comparable to the azurophils of polymorphonuclear leukocytes and the specific granules of eosinophils. Macrophages from peritoneal exudates (72–96 hr after endotoxin injection) contain large quantities of lysosomal enzymes throughout the secretory apparatus (rough ER and Golgi complex), in digestive vacuoles, and in numerous coated vesicles; however, they lack forming or mature azurophil granules. Hence it appears that the monocyte produces two types of primary lysosomes during different phases of its life cycle—azurophil granules made by developing monocytes in bone marrow or blood, and coated vesicles made by macrophages in tissues and body cavities. The Rockefeller University Press 1971-08-01 /pmc/articles/PMC2108281/ /pubmed/4107019 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Nichols, Barbara A.
Bainton, Dorothy Ford
Farquhar, Marilyn G.
DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title_full DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title_fullStr DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title_full_unstemmed DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title_short DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
title_sort differentiation of monocytes : origin, nature, and fate of their azurophil granules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108281/
https://www.ncbi.nlm.nih.gov/pubmed/4107019
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