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DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules
The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108281/ https://www.ncbi.nlm.nih.gov/pubmed/4107019 |
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author | Nichols, Barbara A. Bainton, Dorothy Ford Farquhar, Marilyn G. |
author_facet | Nichols, Barbara A. Bainton, Dorothy Ford Farquhar, Marilyn G. |
author_sort | Nichols, Barbara A. |
collection | PubMed |
description | The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they function as macrophages. Cells were examined from all three phases. It was found that: azurophil granules originate in the Golgi complex of the developing monocyte of bone marrow and blood, and ultimately fuse with phagosomes during phagocytosis upon arrival of monocytes in the tissues. They contain lysosomal enzymes in all species studied and peroxidase in the guinea pig and human. These enzymes are produced by the same pathway as other secretory products (i.e., they are segregated in the rough ER and packaged into granules in the Golgi complex). The findings demonstrate that the azurophil granules of monocytes are primary lysosomes or storage granules comparable to the azurophils of polymorphonuclear leukocytes and the specific granules of eosinophils. Macrophages from peritoneal exudates (72–96 hr after endotoxin injection) contain large quantities of lysosomal enzymes throughout the secretory apparatus (rough ER and Golgi complex), in digestive vacuoles, and in numerous coated vesicles; however, they lack forming or mature azurophil granules. Hence it appears that the monocyte produces two types of primary lysosomes during different phases of its life cycle—azurophil granules made by developing monocytes in bone marrow or blood, and coated vesicles made by macrophages in tissues and body cavities. |
format | Text |
id | pubmed-2108281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21082812008-05-01 DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules Nichols, Barbara A. Bainton, Dorothy Ford Farquhar, Marilyn G. J Cell Biol Article The origin, content, and fate of azurophil granules of blood monocytes were investigated in several species (rabbit, guinea pig, human) by electron microscopy and cytochemistry. The life cycle of monocytes consists of maturation in bone marrow, transit in blood, and migration into tissues where they function as macrophages. Cells were examined from all three phases. It was found that: azurophil granules originate in the Golgi complex of the developing monocyte of bone marrow and blood, and ultimately fuse with phagosomes during phagocytosis upon arrival of monocytes in the tissues. They contain lysosomal enzymes in all species studied and peroxidase in the guinea pig and human. These enzymes are produced by the same pathway as other secretory products (i.e., they are segregated in the rough ER and packaged into granules in the Golgi complex). The findings demonstrate that the azurophil granules of monocytes are primary lysosomes or storage granules comparable to the azurophils of polymorphonuclear leukocytes and the specific granules of eosinophils. Macrophages from peritoneal exudates (72–96 hr after endotoxin injection) contain large quantities of lysosomal enzymes throughout the secretory apparatus (rough ER and Golgi complex), in digestive vacuoles, and in numerous coated vesicles; however, they lack forming or mature azurophil granules. Hence it appears that the monocyte produces two types of primary lysosomes during different phases of its life cycle—azurophil granules made by developing monocytes in bone marrow or blood, and coated vesicles made by macrophages in tissues and body cavities. The Rockefeller University Press 1971-08-01 /pmc/articles/PMC2108281/ /pubmed/4107019 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Nichols, Barbara A. Bainton, Dorothy Ford Farquhar, Marilyn G. DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title | DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title_full | DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title_fullStr | DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title_full_unstemmed | DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title_short | DIFFERENTIATION OF MONOCYTES : Origin, Nature, and Fate of Their Azurophil Granules |
title_sort | differentiation of monocytes : origin, nature, and fate of their azurophil granules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108281/ https://www.ncbi.nlm.nih.gov/pubmed/4107019 |
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