ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana

Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana. The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation. The antisera to tadpole and frog hemoglobins did not cross-react wi...

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Autores principales: Maniatis, George M., Ingram, Vernon M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108329/
https://www.ncbi.nlm.nih.gov/pubmed/19866766
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author Maniatis, George M.
Ingram, Vernon M.
author_facet Maniatis, George M.
Ingram, Vernon M.
author_sort Maniatis, George M.
collection PubMed
description Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana. The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation. The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin. The anti-serum against frog hemoglobin was used for the detection of frog hemoglobin in tadpoles undergoing either natural or thyroxine-induced metamorphosis. It was shown that frog hemoglobin is detectable first in the liver, indicating that the liver is the site of erythrocyte maturation during metamorphosis.
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spelling pubmed-21083292008-05-01 ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana Maniatis, George M. Ingram, Vernon M. J Cell Biol Article Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana. The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation. The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin. The anti-serum against frog hemoglobin was used for the detection of frog hemoglobin in tadpoles undergoing either natural or thyroxine-induced metamorphosis. It was shown that frog hemoglobin is detectable first in the liver, indicating that the liver is the site of erythrocyte maturation during metamorphosis. The Rockefeller University Press 1971-05-01 /pmc/articles/PMC2108329/ /pubmed/19866766 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Maniatis, George M.
Ingram, Vernon M.
ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title_full ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title_fullStr ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title_full_unstemmed ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title_short ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS : II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
title_sort erythropoiesis during amphibian metamorphosis : ii. immunochemical study of larval and adult hemoglobins of rana catesbeiana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108329/
https://www.ncbi.nlm.nih.gov/pubmed/19866766
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