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LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1971
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108335/ https://www.ncbi.nlm.nih.gov/pubmed/19866772 |
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author | Kuettner, K. E. Eisenstein, R. Soble, L. W. Arsenis, C. |
author_facet | Kuettner, K. E. Eisenstein, R. Soble, L. W. Arsenis, C. |
author_sort | Kuettner, K. E. |
collection | PubMed |
description | The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described. |
format | Text |
id | pubmed-2108335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1971 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21083352008-05-01 LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization Kuettner, K. E. Eisenstein, R. Soble, L. W. Arsenis, C. J Cell Biol Article The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described. The Rockefeller University Press 1971-05-01 /pmc/articles/PMC2108335/ /pubmed/19866772 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Kuettner, K. E. Eisenstein, R. Soble, L. W. Arsenis, C. LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title | LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title_full | LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title_fullStr | LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title_full_unstemmed | LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title_short | LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization |
title_sort | lysozyme in epiphyseal cartilage : iv. embryonic chick cartilage lysozyme—its localization and partial characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108335/ https://www.ncbi.nlm.nih.gov/pubmed/19866772 |
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