Cargando…

LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization

The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was...

Descripción completa

Detalles Bibliográficos
Autores principales: Kuettner, K. E., Eisenstein, R., Soble, L. W., Arsenis, C.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108335/
https://www.ncbi.nlm.nih.gov/pubmed/19866772
_version_ 1782139024290349056
author Kuettner, K. E.
Eisenstein, R.
Soble, L. W.
Arsenis, C.
author_facet Kuettner, K. E.
Eisenstein, R.
Soble, L. W.
Arsenis, C.
author_sort Kuettner, K. E.
collection PubMed
description The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.
format Text
id pubmed-2108335
institution National Center for Biotechnology Information
language English
publishDate 1971
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21083352008-05-01 LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization Kuettner, K. E. Eisenstein, R. Soble, L. W. Arsenis, C. J Cell Biol Article The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described. The Rockefeller University Press 1971-05-01 /pmc/articles/PMC2108335/ /pubmed/19866772 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Kuettner, K. E.
Eisenstein, R.
Soble, L. W.
Arsenis, C.
LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title_full LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title_fullStr LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title_full_unstemmed LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title_short LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme—Its Localization and Partial Characterization
title_sort lysozyme in epiphyseal cartilage : iv. embryonic chick cartilage lysozyme—its localization and partial characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108335/
https://www.ncbi.nlm.nih.gov/pubmed/19866772
work_keys_str_mv AT kuettnerke lysozymeinepiphysealcartilageivembryonicchickcartilagelysozymeitslocalizationandpartialcharacterization
AT eisensteinr lysozymeinepiphysealcartilageivembryonicchickcartilagelysozymeitslocalizationandpartialcharacterization
AT soblelw lysozymeinepiphysealcartilageivembryonicchickcartilagelysozymeitslocalizationandpartialcharacterization
AT arsenisc lysozymeinepiphysealcartilageivembryonicchickcartilagelysozymeitslocalizationandpartialcharacterization