STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions

A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes co...

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Detalles Bibliográficos
Autores principales: Nozawa, Yoshinori, Thompson, Guy A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108490/
https://www.ncbi.nlm.nih.gov/pubmed/4933471
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author Nozawa, Yoshinori
Thompson, Guy A.
author_facet Nozawa, Yoshinori
Thompson, Guy A.
author_sort Nozawa, Yoshinori
collection PubMed
description A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.
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spelling pubmed-21084902008-05-01 STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions Nozawa, Yoshinori Thompson, Guy A. J Cell Biol Article A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition. Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes. The Rockefeller University Press 1971-06-01 /pmc/articles/PMC2108490/ /pubmed/4933471 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Nozawa, Yoshinori
Thompson, Guy A.
STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title_full STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title_fullStr STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title_full_unstemmed STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title_short STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS : II. Isolation and Lipid Analysis of Cell Fractions
title_sort studies of membrane formation in tetrahymena pyriformis : ii. isolation and lipid analysis of cell fractions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108490/
https://www.ncbi.nlm.nih.gov/pubmed/4933471
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