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MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES

The fine structure of ascitic cells, consisting of 87–92% heterophil, 5–10% eosinophil leukocytes, and 3% macrophages, is well preserved by glutaraldehyde-osmium tetroxide fixation only when the osmolality of the fixative is appropriately balanced. The β-glycogen particles, 35–45 mµ in diameter, are...

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Autores principales: Wanson, Jean-Claude, Tielemans, Louis
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1971
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108492/
https://www.ncbi.nlm.nih.gov/pubmed/4103956
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author Wanson, Jean-Claude
Tielemans, Louis
author_facet Wanson, Jean-Claude
Tielemans, Louis
author_sort Wanson, Jean-Claude
collection PubMed
description The fine structure of ascitic cells, consisting of 87–92% heterophil, 5–10% eosinophil leukocytes, and 3% macrophages, is well preserved by glutaraldehyde-osmium tetroxide fixation only when the osmolality of the fixative is appropriately balanced. The β-glycogen particles, 35–45 mµ in diameter, are found either as large accumulations in the perinuclear region or in a dispersed form in peripheral cytoplasm. In the heterophils, they are embedded in a coarse-grained ground substance. Extraction and purification of the glycogen were performed by differential precipitation-centrifugation. Yield (35% recovery), purity (4% protein contamination), and preservation of a high sedimentation coefficient (240S) represent the main advantages of the proposed procedure. The analysis of the profile of the sedimentation curve, together with an analysis of the particle size measurements and of particle fine structure, leads to the conclusion that the β-particles form a homogeneous population with a gaussian distribution curve. Each particle consists of smaller units which increase in number with increasing size, the largest ones taking on the appearance of small rosettes. The glycogen particles of the microsomal fraction, still loaded with phosphorylase, were submitted to a synthetic activity by incubation in the presence of glucose-l-phosphate. The analysis of the particle growth shows that particles of all sizes respond equally well.
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spelling pubmed-21084922008-05-01 MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES Wanson, Jean-Claude Tielemans, Louis J Cell Biol Article The fine structure of ascitic cells, consisting of 87–92% heterophil, 5–10% eosinophil leukocytes, and 3% macrophages, is well preserved by glutaraldehyde-osmium tetroxide fixation only when the osmolality of the fixative is appropriately balanced. The β-glycogen particles, 35–45 mµ in diameter, are found either as large accumulations in the perinuclear region or in a dispersed form in peripheral cytoplasm. In the heterophils, they are embedded in a coarse-grained ground substance. Extraction and purification of the glycogen were performed by differential precipitation-centrifugation. Yield (35% recovery), purity (4% protein contamination), and preservation of a high sedimentation coefficient (240S) represent the main advantages of the proposed procedure. The analysis of the profile of the sedimentation curve, together with an analysis of the particle size measurements and of particle fine structure, leads to the conclusion that the β-particles form a homogeneous population with a gaussian distribution curve. Each particle consists of smaller units which increase in number with increasing size, the largest ones taking on the appearance of small rosettes. The glycogen particles of the microsomal fraction, still loaded with phosphorylase, were submitted to a synthetic activity by incubation in the presence of glucose-l-phosphate. The analysis of the particle growth shows that particles of all sizes respond equally well. The Rockefeller University Press 1971-06-01 /pmc/articles/PMC2108492/ /pubmed/4103956 Text en Copyright © 1971 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Wanson, Jean-Claude
Tielemans, Louis
MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title_full MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title_fullStr MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title_full_unstemmed MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title_short MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF GLYCOGEN PARTICLES ISOLATED FROM RABBIT POLYMORPHONUCLEAR LEUKOCYTES
title_sort morphological and biochemical characteristics of glycogen particles isolated from rabbit polymorphonuclear leukocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108492/
https://www.ncbi.nlm.nih.gov/pubmed/4103956
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AT tielemanslouis morphologicalandbiochemicalcharacteristicsofglycogenparticlesisolatedfromrabbitpolymorphonuclearleukocytes