STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS

A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving ce...

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Detalles Bibliográficos
Autores principales: Leffert, H. L., Paul, D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108652/
https://www.ncbi.nlm.nih.gov/pubmed/4333210
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author Leffert, H. L.
Paul, D.
author_facet Leffert, H. L.
Paul, D.
author_sort Leffert, H. L.
collection PubMed
description A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.
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spelling pubmed-21086522008-05-01 STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS Leffert, H. L. Paul, D. J Cell Biol Article A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration. The Rockefeller University Press 1972-03-01 /pmc/articles/PMC2108652/ /pubmed/4333210 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Leffert, H. L.
Paul, D.
STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title_full STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title_fullStr STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title_full_unstemmed STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title_short STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLS
title_sort studies on primary cultures of differentiated fetal liver cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108652/
https://www.ncbi.nlm.nih.gov/pubmed/4333210
work_keys_str_mv AT lefferthl studiesonprimaryculturesofdifferentiatedfetallivercells
AT pauld studiesonprimaryculturesofdifferentiatedfetallivercells

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