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A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS

The fusion of myogenic cells has been examined on the fine-structural level in muscle cell cultures of embryonic Japanese Coturnix quail. Cells, selected by light microscopy, were serially sectioned normal to their long axis. In this plane, oblique sections of cell membranes are rare and plasmalemma...

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Detalles Bibliográficos
Autores principales: Lipton, Bruce H., Konigsberg, Irwin R.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108740/
https://www.ncbi.nlm.nih.gov/pubmed/4554365
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author Lipton, Bruce H.
Konigsberg, Irwin R.
author_facet Lipton, Bruce H.
Konigsberg, Irwin R.
author_sort Lipton, Bruce H.
collection PubMed
description The fusion of myogenic cells has been examined on the fine-structural level in muscle cell cultures of embryonic Japanese Coturnix quail. Cells, selected by light microscopy, were serially sectioned normal to their long axis. In this plane, oblique sections of cell membranes are rare and plasmalemmal profiles are more easily traced between adjacent cells. In seven cases, pairs of cells, apparently fixed in the process of fusion, are joined by a single cytoplasmic bridge. Since obliquely sectioned membranes often suggest cytoplasmic confluence, tilting stage analysis was employed to resolve cell membranes in suspect cases. In contrast to such artifacts of superposition, however, the observed intercommunicating pores are contained within a pair of culs-de-sac formed by the fused membranes of both cells. These blind pouches can be traced back between the cells to the external space. The confluent regions are clearly demarcated and they are not simply areas between vesicular profiles. The results of this analysis suggest that (a) at no time is there any loss of integrity of the cellular envelope, and (b) fusion is most probably initiated at single sites between pairs of cells, the pore enlarging, leaving first vestiges and eventually no trace of the original intervening membranes.
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spelling pubmed-21087402008-05-01 A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS Lipton, Bruce H. Konigsberg, Irwin R. J Cell Biol Article The fusion of myogenic cells has been examined on the fine-structural level in muscle cell cultures of embryonic Japanese Coturnix quail. Cells, selected by light microscopy, were serially sectioned normal to their long axis. In this plane, oblique sections of cell membranes are rare and plasmalemmal profiles are more easily traced between adjacent cells. In seven cases, pairs of cells, apparently fixed in the process of fusion, are joined by a single cytoplasmic bridge. Since obliquely sectioned membranes often suggest cytoplasmic confluence, tilting stage analysis was employed to resolve cell membranes in suspect cases. In contrast to such artifacts of superposition, however, the observed intercommunicating pores are contained within a pair of culs-de-sac formed by the fused membranes of both cells. These blind pouches can be traced back between the cells to the external space. The confluent regions are clearly demarcated and they are not simply areas between vesicular profiles. The results of this analysis suggest that (a) at no time is there any loss of integrity of the cellular envelope, and (b) fusion is most probably initiated at single sites between pairs of cells, the pore enlarging, leaving first vestiges and eventually no trace of the original intervening membranes. The Rockefeller University Press 1972-05-01 /pmc/articles/PMC2108740/ /pubmed/4554365 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Lipton, Bruce H.
Konigsberg, Irwin R.
A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title_full A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title_fullStr A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title_full_unstemmed A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title_short A FINE-STRUCTURAL ANALYSIS OF THE FUSION OF MYOGENIC CELLS
title_sort fine-structural analysis of the fusion of myogenic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108740/
https://www.ncbi.nlm.nih.gov/pubmed/4554365
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